Neuroexcitatory amino acids and their relation to infarct size and neurological deficit in ischemic stroke
Neuroexcitatory amino acids and their relation to infarct size and neurological deficit in ischemic stroke
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DOI：
10.1161/01.str.27.6.1060
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发表时间：
1996-06-01
期刊：
影响因子：
8.3
通讯作者：
Noya, M
中科院分区：
文献类型：
--
作者：
Castillo, J;Davalos, A;Noya, M
背景与目的兴奋性氨基酸（EAAs）参与缺血性神经元病变的发病机制已被实验证实，但临床经验匮乏。我们的目的是检测脑梗塞急性期的 EAA 水平与梗塞面积和神经功能缺损的强度之间的关系。方法采用高效液相色谱法，测定 128 例经 CT 确诊的缺血性脑梗塞患者和 43 名对照受试者血浆和脑脊液 (CSF) 中谷氨酸、天冬氨酸、牛磺酸和甘氨酸的浓度。入院后 24 小时内采集血液和脑脊液样本。在这些测试之后以及纳入研究后 48 小时立即使用加拿大中风量表评估神经功能缺损的严重程度。患者入组后第4天至第7天进行第二次CT检查，确定梗死体积。 结果 对照组血浆谷氨酸浓度为121.39+/-80.89 μmol/L，患者组血浆谷氨酸浓度为163.71+/-103.13 μmol/L（P=.015）；在脑脊液中，对照受试者为 3.46+/-1.20 μmol/L，患者为 6.55+/-4.65 μmol/L（P
Background and Purpose The participation of excitatory amino acids (EAAs) in the pathogenesis of ischemic neuronal lesion has been experimentally demonstrated, but clinical experience is scarce. Our objective was to examine EAA levels during the acute phase of cerebral infarction in relation to infarct size and intensity of neurological deficit.Methods Using high-performance liquid chromatography, we determined the glutamate, aspartate, taurine, and glycine concentrations in the plasma and cerebrospinal fluid (CSF) of 128 patients with ischemic cerebral infarction confirmed by CT and 43 control subjects. Blood and CSF samples were obtained on admission within the first 24 hours from symptom onset. The severity of the neurological deficit was assessed with the Canadian Stroke Scale immediately after these tests and at 48 hours after inclusion in the study. Infarct volume was determined in a second CT performed between the 4th and 7th day after the patient's inclusion.Results The concentration of plasmatic glutamate was 121.39+/-80.89 mu mol/L in the control group and 163.71+/-103.13 mu mol/L in the patient group (P=.015); in CSF it was 3.46+/-1.20 mu mol/L in control subjects and 6.55+/-4.65 mu mol/L in patients (P