Changes of plasma concentrations of insulin-like peptide 3 and testosterone, and their association with scrotal circumference during pubertal development in male goats
Changes of plasma concentrations of insulin-like peptide 3 and testosterone, and their association with scrotal circumference during pubertal development in male goats
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DOI：
10.1016/j.theriogenology.2017.01.009
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发表时间：
2017-04-01
期刊：
影响因子：
2.8
通讯作者：
Tamada, H.
中科院分区：
文献类型：
--
作者：
Hannan, M. A.;Kawate, N.;Tamada, H.
胰岛素样肽3（INSL 3）已被用作几个物种青春期的睾丸特异性生物标志物，但在小反刍动物青春期发育过程中INSL 3的分泌模式尚不清楚。在这里，我们试图确定年龄相关的变化，在血浆中的INSL 3和睾酮浓度和它们的关联与阴囊周长在青春期发育期间在5只雄性芝山羊。从每只山羊出生后第10周至第52周，每2周采集一次血样并测量阴囊周长。根据阴囊周长的变化，将数据分为青春期早期（10-22周）、青春期晚期（22-34周）和青春期后（34-52周）类别。采用酶免疫分析法（ElAs）测定血浆睾酮和黄体生成素（LH）浓度，采用时间分辨荧光免疫分析法（TRFIA）测定血浆INSL 3。两周一次的采样显示，血浆INSL 3分泌在青春期期间和之后保持适度增加，而血浆睾酮分泌在同一时期波动。三个年龄类别的比较显示，与青春期早期相比，青春期晚期和青春期后期间的平均血浆INSL 3浓度显著增加（p < 0.01）。青春期早期和晚期之间的平均血浆睾酮浓度没有差异，但与青春期早期和晚期相比，在青春期后期间观察到显著增加（p < 0.01）。平均血浆LH浓度从青春早期到青春晚期和从青春晚期到青春后期显著增加（p < 0.05）。从青春期早期到青春期晚期以及从青春期晚期到青春期后，观察到平均阴囊周长显著增加（p < 0.05）。在10 ~ 52周龄，阴囊围与INSL 3的最佳回归曲线的R-2值（0.513; p < 0.001）高于阴囊围与睾酮的最佳回归曲线的R-2值（0.162; p < 0.01）。总之，在雄性山羊中，INSL 3的血浆浓度在青春期期间和之后持续增加，而睾酮分泌波动。与睾酮相比，阴囊周长与INSL 3浓度的相关性更高，这意味着INSL 3作为睾丸总Leydig细胞体积的生物标志物是上级的。(C)2017爱思唯尔公司All rights reserved.
Insulin-like peptide 3 (INSL3) has been used as a testis-specific biomarker for puberty in several species, but the secretory profile of INSL3 during pubertal development in small ruminants is unknown. Here we sought to determine the age-related changes in the plasma concentrations of INSL3 and testosterone and their association with scrotal circumference during pubertal development in five male Shiba goats. Blood samples and scrotal circumference measurement were taken every 2 weeks from week 10 to week 52 of each goat's lifespan. Based on the changes in scrotal circumference, data were grouped into early pubertal (10-22 weeks), late pubertal (22-34 weeks) and post-pubertal (34-52 weeks) categories. The plasma concentrations of testosterone and luteinizing hormone (LH) were measured by enzyme immunoassays (ElAs), and we used a time-resolved fluorescence immunoassay (TRFIA) to measure plasma INSL3. The biweekly sampling showed that the plasma INSL3 secretions maintained a moderate increase during and after puberty, whereas the plasma testosterone secretions fluctuated over the same period. The comparison of the three age categories revealed a significant increase (p < 0.01) in the mean plasma INSL3 concentrations during the late and post-pubertal periods compared to the early pubertal period. There was no difference in the mean plasma testosterone concentrations between the early and late pubertal periods, but a significant increase (p < 0.01) was observed during the post-pubertal period compared to early and late pubertal periods. The mean plasma LH concentrations increased significantly (p < 0.05) from the early pubertal to late pubertal and from the late pubertal to post-pubertal periods. A significant increase (p < 0.05) in the mean scrotal circumference from the early pubertal to late pubertal and from the late pubertal to post-pubertal periods was observed. The R-2 value of the best regression curves between scrotal circumference and INSL3 (0.513; p < 0.001) was higher than that between scrotal circumference and testosterone (0.162; p < 0.01) from 10 to 52 weeks of age. In conclusion, in male goats, plasma concentrations of INSL3 increased continuously during and after puberty, whereas testosterone secretions were fluctuated. The scrotal circumference was more highly correlated with the INSL3 concentrations than with testosterone, implying that INSL3 is superior as a biomarker of testicular total Leydig cell volume. (C) 2017 Elsevier Inc. All rights reserved.