Reagentless ultrasensitive specific DNA array detection based on responsive polymeric biochips.
Reagentless ultrasensitive specific DNA array detection based on responsive polymeric biochips.
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DOI：
10.1021/ac061650
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发表时间：
2006-10
影响因子：
7.4
通讯作者：
Ahmed Najari;H. Ho;J. Gravel;Philippe Nobert;D. Boudreau;M. Leclerc
中科院分区：
文献类型：
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作者：
Ahmed Najari;H. Ho;J. Gravel;Philippe Nobert;D. Boudreau;M. Leclerc
研究了固定在固体底物上的荧光标记寡核苷酸探针和光学聚合物传感器组成的自组装分子结构，用于DNA靶分子的痕量水平检测。聚合物换能器和分子聚集体内的荧光团之间快速而有效的能量转移导致了荧光信号的大量本征放大，并实现了对低至300个DNA分子的无标记检测，具有检测单核苷酸错配所需的特异性。当传感结构连接到固体载体上时，这种全分子检测水平的能力可能导致快速和简单的无聚合酶链式反应的多目标DNA检测的生物芯片平台的开发。
Self-assembled molecular structures immobilized on solid substrates and composed of fluorophore-tagged oligonucleotide probes and an optical polymeric transducer were investigated for the trace level detection of DNA target molecules. Rapid and efficient energy transfer between the polymeric transducer and fluorophores within the molecular aggregates leads to a massive intrinsic amplification of the fluorescence signal and to the label-free detection of as little as 300 DNA molecules, with the specificity required for the detection of single-nucleotide mismatches. This capacity for attomolar detection levels while the sensing structures are attached onto solid supports could lead to the development of biochip platforms for fast and simple PCR-free multitarget DNA detection.