A Novel Method for Continuous Formation of Cord-Like Collagen Gels to Fabricate Durable Fibers in Which Collagen Fibrils Are Longitudinally Aligned
A Novel Method for Continuous Formation of Cord-Like Collagen Gels to Fabricate Durable Fibers in Which Collagen Fibrils Are Longitudinally Aligned
复制标题
连续形成绳状胶原蛋白凝胶以制造胶原原纤维纵向排列的耐用纤维的新方法
DOI：
10.1002/jbm.b.34194
复制
发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Yasuda K
中科院分区：
文献类型：
--
作者：
Yunoki S;Hatayama H;Ebisawa M;Kondo E;Yasuda K
我们开发了一种连续形成索状胶原凝胶的方法，该凝胶包含优先沿几何轴（CCG）沿着的原纤维。将溶解在含有280 mM氯化钠的磷酸钠缓冲液中的胶原蛋白（2.5%）以2.5 mm/s的线速度引入到在38°C下加热的不锈钢圆筒（长52 mm，直径2.0 mm）中。该过程导致在圆柱体中急性原纤维形成下胶原原纤维排列，导致连续形成CCG。原纤形成速率、剪切速率和剪切持续时间是成功形成CCG的重要因素。与传统湿法纺丝相比，该方法的突出优点包括：该技术能够在整个凝胶中形成对齐的原纤维，并将绳状凝胶的直径控制在1 mm以上。将与1-乙基-3-（3-二甲基氨基丙基）-碳二亚胺和N-羟基-琥珀酰亚胺交联的CCG风干，生产出横截面积为0.0123-0.135 mm 2的干胶原纤维。在纤维再润湿时，它们在54.5 ± 7.8 MPa的应力下失效，这高于前交叉韧带组织的平均失效应力（13.3-37.8 MPa）。这些发现表明，CCG形成方法能够制造胶原纤维，胶原纤维是胶原基人工肌腱的潜在成分。© 2018 Wiley Periodicals，Inc. J Biomed Mater Res Part B：Appl Biomater 107 B：1011-1023，2019。
We developed a continuous formation method of cord‐like collagen gels comprising fibrils preferentially aligned along the geometrical axes (CCGs). Collagen (2.5%) dissolved in a sodium phosphate buffer containing 280 mMof sodium chloride was introduced into a stainless cylinder (length 52 mm, diameter 2.0 mm) warmed at 38°C at a linear velocity of 2.5 mm/s. This process caused collagen fibril alignments under acute fibril formation in the cylinder, resulting in continuous formation of CCGs. Fibril formation rate, shear rate, and shear duration were substantial factors for successful CCG formation. Outstanding advantages of this method over conventional wet spinning include the capacity of this technique to form aligned fibrils in the entire gels and to control the diameter of cord‐like gels over 1 mm. The air‐drying of CCGs which were crosslinked with 1‐ethyl‐3‐(3‐dimethylaminopropyl)‐carbodiimide and N‐hydroxy‐succinimide produced dry collagen fibers with cross‐sectional areas of 0.0123–0.135 mm2. Upon the rewetting of the fibers, they failed at a stress of 54.5 ± 7.8 MPa, which is higher than the mean failure stress of anterior cruciate ligament tissue (13.3–37.8 MPa). These findings indicate that the CCG formation method enables the fabrication of collagen fibers which are potential components of collagen‐based artificial tendons. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1011–1023, 2019.