Identification of Pirh2D, an Additional Novel Isoform of Pirh2 Ubiquitin Ligase.
Identification of Pirh2D, an Additional Novel Isoform of Pirh2 Ubiquitin Ligase.
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DOI：
10.4255/mcpharmacol.10.04
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Sheikh MS
中科院分区：
文献类型：
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作者：
Shi J;Huang Y;Sheikh MS
Pirh2是一种E3泛素连接酶，可促进肿瘤抑制因子p53泛素化和蛋白酶体降解。最近，我们报道了两种新的Pirh2亚型Pirh2B和Pirh2C的鉴定和表征，并因此将全长Pirh2重新分类为Pirh2A。Pirh2B和C都负调节p53，并且还表现出与MDM2的相互作用。在这里，我们报告存在一个额外的Pirh2亚型，我们命名为Pirh2D。Pirh2D是一个由75个氨基酸组成的截短型蛋白质，其氨基端氨基酸序列与Pirh2A、Pirh2B和Pirh2C相同，羧基端氨基酸序列与Pirh2A、Pirh2B和Pirh2C相同，而羧基端氨基酸序列与Pirh2A、Pirh2B和Pirh2C相同。需要进一步的研究来确定Pirh2D是否也以类似于Pirh2B和Pirh2C的方式起作用。
Pirh2 is an E3 ubiquitin ligase that promotes tumor suppressor p53 ubiquitination and proteasomal degradation. Recently, we have reported the identification and characterization of two novel isoforms of Pirh2 named Pirh2B and Pirh2C and accordingly, reclassified the full-length Pirh2 as Pirh2A. Both Pirh2B and C negatively regulate p53 and also exhibit interactions with MDM2. Here, we report the existence of an additional Pirh2 isoform that we have named Pirh2D. Translation of nucleotide sequence predicts Pirh2D to be composed of 75 amino acids with a molecular mass of 8493.74 Da. Thus, Pirh2D is a truncated protein that harbors 67 amino-terminal amino acids identical to those in Pirh2A, Pirh2B and Pirh2C and has 8 additional unique amino acids at the carboxyl-terminal end. Further studies are needed to determine whether Pirh2D also functions in a manner similar to Pirh2B and Pirh2C.