The Genetic Architecture of Fluctuating Asymmetry of Mandible Size and Shape in a Population of Mice: Another Look
The Genetic Architecture of Fluctuating Asymmetry of Mandible Size and Shape in a Population of Mice: Another Look
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DOI：
10.3390/sym7010146
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发表时间：
2015-02
期刊：
影响因子：
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通讯作者：
L. Leamy;C. Klingenberg;E. Sherratt;J. Wolf;J. Cheverud
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文献类型：
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作者：
L. Leamy;C. Klingenberg;E. Sherratt;J. Wolf;J. Cheverud
波动不对称(FA)通常由左右两侧性状的差异来衡量，通常用于评估人群中的发育不稳定(DI)。先前使用F2杂交小鼠群体进行的数量性状基因座(QTL)研究发现，几乎没有证据表明个别基因座影响下颌大小的FA，但基因座之间存在大量的上位性交互作用。在这里，我们通过测试这些模式是否在来自同一杂交的F3群体中复制来扩展这项工作。利用在1200多只小鼠中进行基因分型的大量分子标记，我们发现影响下颌骨大小(和形状)的基因座(QTL)之间存在显著的交互作用。上位性仅在0.0001概率水平上就贡献了大约20%的FASIZE变异和19%的FASHAPE变异，与先前对F2小鼠的估计值相当，而且比影响下颌骨FA性状的少数单基因座QTL产生的QTL大得多。我们发现的FA的单基因座和上位性QTL的位置表明，DI的合理候选基因是那些控制性状本身大小或形状的基因，它们可能与热休克蛋白基因相互作用。
Fluctuating asymmetry (FA), typically measured by variation in the differences between right and left sides of bilateral traits, is commonly used to assess developmental instability (DI) in populations. A previous quantitative trait locus (QTL) investigation using an F2 intercross mouse population found little evidence of individual loci affecting FA in mandible size, but an abundance of epistatic interactions between loci. Here we extend this work by testing whether these patterns replicate in an F3 population derived from the same intercross. Using a large number of molecular markers genotyped in over 1200 mice, we uncovered significant interactions between loci (QTLs) affecting FA in mandible size (and shape). Epistasis contributed roughly 20% of the variation in FASIZE and 19% of the variation in FASHAPE at the 0.0001 probability level alone, and was comparable to that previously estimated for the F2 mice, and much greater than that generated from the few single-locus QTLs affecting the mandible FA traits. The positions of the single-locus and epistatic QTLs for FA that we discovered suggested that logical candidate genes for DI are those controlling size or shape in the traits themselves, and that they may be interacting with genes for heat shock proteins.