Enterococcal surface protein esp is important for biofilm formation of Enterococcus faecium E1162
Enterococcal surface protein esp is important for biofilm formation of Enterococcus faecium E1162
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DOI：
10.1128/jb.01205-07
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发表时间：
2007-11-01
影响因子：
3.2
通讯作者：
Willems, Rob J. L.
中科院分区：
文献类型：
--
作者：
Heikens, Esther;Bonten, Marc J. M.;Willems, Rob J. L.
肠球菌已成为重要的医院病原体，对多种抗生素具有耐药性。粘附于非生物材料和在医疗器械上形成生物膜被认为是重要的毒力特性。屎肠球菌复合体17（Enterococcus faecium complex 17，CC 17）是一个单一的克隆谱系，是一种成功的院内致病菌，大多数CC 17分离株都携带肠球菌表面蛋白基因，尤其是esp基因。CC 17菌株。此外，进行初始粘附和生物膜测定。与野生型菌株相比，ESP插入-缺失突变体不再在细胞表面上产生ESP，并且具有显著较低的对聚苯乙烯的初始粘附和显著较少的生物膜形成，导致生物膜水平与ESP阴性分离株的生物膜水平相当。通过与ESP的反式互补，在插入-缺失突变体中恢复了初始粘附和生物膜形成的能力。这些结果确定Esp为E.屎肠菌CC 17在生物膜形成中具有重要作用，生物膜形成是感染发病的重要因素。
Enterococci have emerged as important nosocomial pathogens with resistance to multiple antibiotics. Adhesion to abiotic materials and biofilm formation on medical devices are considered important virulence properties. A single clonal lineage of Enterococcus faecium, complex 17 (CC17), appears to be a successful nosocomial pathogen, and most CC17 isolates harbor the enterococcal surface protein gene, esp. In this study, we constructed an esp insertion-deletion mutant in a clinical E. faecium CC17 isolate. In addition, initial adherence and biofilm assays were performed. Compared to the wild-type strain, the esp insertion-deletion mutant no longer produced Esp on the cell surface and had significantly lower initial adherence to polystyrene and significantly less biofilm formation, resulting in levels of biofilm comparable to those of an esp-negative isolate. Capacities for initial adherence and biofilm formation were restored in the insertion-deletion mutant by in trans complementation with esp. These results identify Esp as the first documented determinant in E. faecium CC17 with an important role in biofilm formation, which is an essential factor in infection pathogenesis.