Rapid modifications of somatostatin neuron activity in the periventricular nucleus after acute stress
Rapid modifications of somatostatin neuron activity in the periventricular nucleus after acute stress
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DOI：
10.1007/s002210000462
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发表时间：
2000-09-01
影响因子：
2
通讯作者：
Armario, A
中科院分区：
文献类型：
--
作者：
Arancibia, S;Rage, F;Armario, A
我们以前曾报道，应激诱导下丘脑生长抑素（SS）的释放迅速增加。在目前的工作中，我们研究了SS的合成是否也受到这种治疗。雄性大鼠进行了15分钟的制动（IMO）的压力，并测量SS mRNA水平和SS mRNA的细胞进行了分析，在室周核（PeV）的放射性和非放射性原位杂交（ISH），分别。用放射免疫分析法（RIA）和北方印迹法（Northern blot）分别测定下丘脑SS含量和总SSmRNA。通过将放射性标记的（S-35）或地高辛（DIG）标记的寡核苷酸探针应用于含有下丘脑前区（AHA）脑室周围区域的组织切片上进行ISH。ISI-I分析表明，在应激大鼠SS mRNA水平显着增加。与此相反，压力处理减少了35%，与相同的组织切片从幼稚对照大鼠在这个区域中的地高辛标记的细胞表达SS mRNA的数量。此外，观察到总SS mRNA地高辛标记面积显著减少。最后，与对照组大鼠相比，应激大鼠整个下丘脑中SS含量和SS mRNA均受到明显抑制。我们的数据表明，IMO应激诱导PeV-AHA中SS mRNA水平显着且快速增加，同时表达SS mRNA的细胞数量减少。目前的研究结果表明，PeV SS神经元的一个子集，这成为沉默的压力发作时，独立于其余的整个质量的PeV神经元的调节。这种差异控制与室周SS产生神经元中描述的细胞异质性以及分配给SS的多个下丘脑和垂体功能一致。
We have previously reported that stress induces a rapid increase in hypothalamic somatostatin (SS) release. In the present work, we investigated whether SS synthesis is also affected by this treatment. Male rats were subjected to 15-min immobilization (IMO) stress, and measurements of both SS mRNA levels and SS mRNA-containing cells were analyzed in the periventricular nucleus (PeV) by radioactive and nonradioactive in situ hybridization (ISH), respectively. In addition, SS content and total SS mRNA were measured in the whole hypothalamus by radioimmunoassay (RIA) and northern blot analysis, respectively. ISH was conducted by applying either a radioactive-labeled (S-35) or a digoxigenin (DIG)-labeled oligonucleotide probe on histological sections containing the periventricular region of the anterior hypothalamic area (AHA). ISI-I analysis using radioactive label showed a significant increase in SS mRNA levels in stressed rats. In contrast, stress treatment decreased the number of DIG-labeled cells expressing SS mRNA in this region by 35% as compared to the same histological sections from naive control rats. In addition, a significant decrease in the total SS mRNA DIG-labeled area was observed. Finally, SS content and SS mRNA measured in the whole hypothalamus of stressed rats were markedly inhibited as compared to control rats. Our data show that IMO stress induces a significant and rapid increase in SS mRNA level accompanied by a decrease in the number of cells expressing SS mRNA in the PeV-AHA. The present results suggest that a subset of PeV SS neurons, which became silent at the onset of stress, are regulated independently of the remaining whole mass of PeV neurons. This differential control is in line with the cellular heterogeneity described in periventricular SS-producing neurons and with the multiple hypothalamic and pituitary functions assigned to SS.