PRODUCTION OF SOMATIC HYBRIDS BY PROTOPLAST FUSION IN MOSS, PHYSCOMITRELLA-PATENS
PRODUCTION OF SOMATIC HYBRIDS BY PROTOPLAST FUSION IN MOSS, PHYSCOMITRELLA-PATENS
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DOI：
10.1007/bf00265582
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发表时间：
1977-01-01
期刊：
影响因子：
--
通讯作者：
COVE, DJ
中科院分区：
文献类型：
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作者：
GRIMSLEY, NH;ASHTON, NW;COVE, DJ
开发了一种从扁豆原生质体组织中分离大量原生质体并将其再生为完整植株的技术。在高pH条件下，用50 mM CaCl2处理来自互补营养缺陷型菌株的原生质体混合物，筛选出体细胞杂种。杂交种的形态与普通单倍体不同，但与无孢子产生的二倍体相似。杂种自交产生的后代表现出分离，这与它们是同源四倍体减数分裂的产物是一致的。
A technique was developed for the isolation of large numbers of protoplasts from protonemal tissue of P. patens, and for their regeneration to give whole plants. Somatic hybrids were selected following treatment of mixtures of protoplasts from complementary auxotrophic strains with 50 mM CaCl2 at high pH. The hybrids have a morphology different from that of normal haploid strains, but similar to that of aposporously produced diploids. The progeny resulting from self-fertilization of the hybrids show a segregation which is consistent with their being the products of meioses in an autotetraploid.