Salinicoccus salitudinis sp nov., a new moderately halophilic bacterium isolated from a saline soil sample
Salinicoccus salitudinis sp nov., a new moderately halophilic bacterium isolated from a saline soil sample
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Salinicoccus salitudinis sp nov.，从盐渍土壤样品中分离出的一种新的中度嗜盐细菌
DOI：
10.1007/s00792-007-0116-8
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发表时间：
2008-03-01
期刊：
影响因子：
2.9
通讯作者：
Jiang, Cheng-Lin
中科院分区：
文献类型：
--
作者：
Chen, Yi-Guang;Cui, Xiao-Long;Jiang, Cheng-Lin
从中国西北部柴达木盆地高盐碱地采集的盐渍土壤样品中分离到一株新的淡黄色素、中度嗜盐、兼性嗜碱、不能运动、不形成孢子、过氧化氢酶和氧化酶阳性的革兰氏阳性球菌YIM-C678(T)。该菌在4~37℃、pH 6.0~11.0范围内生长，最适生长温度为25℃、pH为8.0。菌株YIM-C678(T)在10-12%(w/v)的氯化钠中生长最好，在1-25%(w/v)的氯化钠中生长最好。细胞壁粘质素型为L-赖氨酸-甘氨酸(5)。细胞内主要脂肪酸为Anteiso-C-15：0、iso-C-15：0、iso-C-16：0和C-16：0。MK-6是主要的呼吸对苯二酚。DNA G+C含量为46.5mol%。基于16SRRNA基因序列的系统发育分析表明，菌株YIM-C678(T)属于葡萄球菌科，与已描述的8种盐生真菌的亲缘关系最近，序列相似性为92.2%(S.luteus Yim 70202(T))至97.5%(S.kunmingensis Yim YimY15(T))。菌株Yim-C678(T)与Kunmingensis Yim Y15(T)的DNA-DNA同源性为35.4%。化学分类数据和16S rRNA基因序列分析支持菌株Yim-C678(T)与盐霉属的亲缘关系。结合系统发育分析、表型特征、化学分类差异和DNA-DNA杂交数据，支持该细菌代表盐藻属一个新种的观点，命名为Salinicoccus salitudinis sp.11月YIM-C678(T)(=DSM 17846=CGMCC 1.6299)为模式菌株。
A novel pale-yellow-pigmented, moderately halophilic, facultatively alkaliphilic, non-motile, non-spore-forming, catalase- and oxidase-positive, obligately aerobic Gram-positive coccus, strain YIM-C678(T) was isolated from a saline soil sample collected from a hypersaline habitat in the Qaidam basin, northwest China. The organism grew at 4-37 degrees C and pH 6.0-11.0, with optimum growth at 25 degrees C and pH 8.0. Strain YIM-C678(T) grew optimally in the presence of 10-12% (w/v) NaCl and growth was observed in 1-25% (w/v) NaCl. The cell wall murein type was L-Lys-Gly(5). Major cellular fatty acids were anteiso-C-15:0, iso-C-15:0, iso-C-16:0 and C-16:0. Menaquinone 6 (MK-6) was the major respiratory quinone. The DNA G + C content was 46.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain YIM-C678(T) belonged to the family Staphylococcaceae and was most closely related to the eight described species of the genus Salinicoccus with sequence similarities from 92.2 (S. luteus YIM 70202(T)) to 97.5% (S. kunmingensis YIM Y15(T)). The DNA-DNA relatedness between strain YIM-C678(T) and S. kunmingensis YIM Y15(T) was 35.4%. Chemotaxonomic data and 16S rRNA gene sequence analysis supported the affiliation of strain YIM-C678(T) with the genus Salinicoccus. The combination of phylogenetic analysis, phenotypic characteristics, chemotaxonomic differences and DNA-DNA hybridization data supported the view that the bacterium represents a novel species of the genus Salinicoccus, for which the name Salinicoccus salitudinis sp. nov. is proposed, with YIM-C678(T) (=DSM 17846 = CGMCC 1.6299) as the type strain.