A scoring system for the evaluation of the mutated Crb1/rd8-derived retinal lesions in C57BL/6N mice.
A scoring system for the evaluation of the mutated Crb1/rd8-derived retinal lesions in C57BL/6N mice.
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DOI：
10.12688/f1000research.11252.1
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发表时间：
2017-01-01
期刊：
影响因子：
--
通讯作者：
Wells, Sara
中科院分区：
文献类型：
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作者：
Concas, Danilo;Cater, Heather;Wells, Sara
作为国际小鼠表型鉴定联盟（IMPC）计划的一部分，MRC Harwell正在对转基因小鼠进行大眼睛形态表型筛选，与在C57 BL/6 NTac背景品系中观察到的基线表型进行比较。已知C57 BL/6 NTac菌株在Crb 1基因中携带自发突变，该突变导致视网膜变性，其特征在于眼底存在白色斑点（flaps）。这些波动可能代表一个混杂因素，掩盖了类似的视网膜表型异常，可能会在突变体中检测到。因此，我们研究了在大量C57 BL/6 NTac小鼠中florescence的频率、位置和程度，为评估具有相同遗传背景的突变小鼠中florescence的存在提供基础。我们发现，在我们的设施，男性比女性更严重的影响，在男性和女性的最常见的定位的florescence是在下半脑的眼底。
As part of the International Mouse Phenotyping Consortium (IMPC) programme, the MRC Harwell is conducting a large eye morphology phenotyping screen on genetically modified mice compared to the baseline phenotype observed in the background strain of C57BL/6NTac. The C57BL/6NTac strain is known to carry a spontaneous mutation in the Crb1 gene that causes retinal degeneration characterized by the presence of white spots (flecks) in the fundus. These flecks potentially represent a confounding factor, masking similar retinal phenotype abnormalities that may be detected in mutants. Therefore we investigated the frequency, position and extent of the flecks in a large population of C57BL/6NTac mice to provide the basis for evaluating the presence of flecks in mutant mice with the same genetic background. We found that in our facility males were more severely affected than females and that in both males and females the most common localisation of the flecks was in the inferior hemicycle of the fundus.