EXPRESSION OF HUMAN-CD4 IN TRANSGENIC MICE DOES NOT CONFER SENSITIVITY TO HUMAN-IMMUNODEFICIENCY-VIRUS INFECTION
EXPRESSION OF HUMAN-CD4 IN TRANSGENIC MICE DOES NOT CONFER SENSITIVITY TO HUMAN-IMMUNODEFICIENCY-VIRUS INFECTION
复制标题
DOI：
10.1089/aid.1992.8.2063
复制
发表时间：
1992-12-01
影响因子：
1.5
通讯作者：
WEILL, JC
中科院分区：
文献类型：
--
作者：
LORES, P;BOUCHER, V;WEILL, JC
将人CD 4分子转染到小鼠细胞中并不赋予对人类免疫缺陷病毒1型（HIV-1）感染的易感性。10在转基因小鼠中表达人CD 4分子被认为提供了一些新的可能性。然而，转基因小鼠T细胞表达的人CD 4受体，或混合的人/小鼠CD 4受体单独或与人主要组织相容性复合体I类分子，是难治的体外HIV-1感染。此外，在体内将HIV接种到这些不同转基因系的小鼠中后，未观察到感染。向转基因小鼠注射重组gp 160病毒蛋白不会改变其T和B细胞群。在小鼠细胞中，防止艾滋病毒进入的显性块的存在进行了讨论。
Transfection of the human CD4 molecule into mouse cells does not confer susceptibility to human immunodeficiency virus type 1 (HIV-1) infection.10 Expression of the human CD4 molecule in transgenic mice was seen to offer some new possibilities. However, transgenic mouse T cells expressing either the human CD4 receptor, or a hybrid human/mouse CD4 receptor alone or in conjunction with human major histocompatibility complex class I molecules, were refractory to in vitro HIV-1 infection. In addition, no infection was observed after in vivo HIV inoculation to mice of these various transgenic lines. Injection of recombinant gp160 viral protein to the transgenic mice did not alter their T and B cell populations. The existence of a dominant block in mouse cells that prevents HIV entry is discussed.