INHIBITION OF THE INFECTIVITY OF INFLUENZA-VIRUS BY TEA POLYPHENOLS
INHIBITION OF THE INFECTIVITY OF INFLUENZA-VIRUS BY TEA POLYPHENOLS
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DOI：
10.1016/0166-3542(93)90008-7
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发表时间：
1993-08-01
影响因子：
7.6
通讯作者：
SHIMAMURA, T
中科院分区：
文献类型：
--
作者：
NAKAYAMA, M;SUZUKI, K;SHIMAMURA, T
（-）表没食子儿茶素没食子酸酯（EGCg）和茶黄素没食子酸酯（TF 3）（1-10 μ M）在体外抑制A型流感病毒和B型流感病毒在Madin-Darby犬肾（MDCK）细胞中的感染性。电子显微镜研究表明，EGCg和TF 3（1 mM）与抗体一样凝集流感病毒，并阻止病毒吸附到MDCK细胞上。EGCg和TF 3更弱地抑制病毒吸附到MDCK细胞。EGCg和TF 3（1-16 μ M）也抑制流感病毒的血凝。这些发现表明茶多酚与流感病毒的血凝素结合，抑制其吸附到MDCK细胞，从而阻断其感染性。
(-)Epigallocatechin gallate (EGCg) and theaflavin digallate (TF3) (1-10 muM) inhibited the infectivity of both influenza A virus and influenza B virus in Madin-Darby canine kidney (MDCK) cells in vitro. Study by electron microscope revealed that EGCg and TF3 (1 mM) agglutinated influenza viruses as well as did antibody, and that they prevented the viruses from adsorbing to MDCK cells. EGCg and TF3 more weakly inhibited adsorption of the viruses to MDCK cells. EGCg and TF3 (1-16 muM) also inhibited haemagglutination by influenza viruses. These findings suggest that tea polyphenols bind to the haemagglutinin of influenza virus, inhibit its adsorption to MDCK cells, and thus block its infectivity.