Thermal imaging and carbon isotope composition indicate variation amongst strawberry (Fragaria x ananassa) cultivars in stomatal conductance and water use efficiency
Thermal imaging and carbon isotope composition indicate variation amongst strawberry (Fragaria x ananassa) cultivars in stomatal conductance and water use efficiency
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DOI：
10.1016/j.envexpbot.2011.09.013
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发表时间：
2012-02-01
影响因子：
5.7
通讯作者：
Simpson, David W.
中科院分区：
文献类型：
--
作者：
Grant, Olga M.;Davies, Michael J.;Simpson, David W.
气孔导度 (g(s)) 较低的品种在干旱条件下可能表现出较高的水分利用效率 (WUE)，但在最佳条件下，低 g(s) 可能会导致活力较低。热成像和碳同位素成分 (δ C-13) 分析的结合提供了同时筛选高 g(s) 和高 WUE 的潜力。十个草莓品种（Fragaria X ananassa Duch.）在浇水良好或水分有限的条件下生长。采用各种方法拍摄植物的热图像，以确定检测 g(s) 变化的最佳方案，包括使用参考叶子、保持叶子水平的网格以及与热图像同步收集气象数据。测定了叶子、果实和树冠的 delta C-13。由水平叶片温度和干参考温度得出的 g(s) 指数显示品种之间以及浇水良好和水分有限的植物之间存在显着差异，根据叶片温度、干参考温度和湿度估算的 g(s) 也是如此。热成像显示“Elsanta”和“Totem”的 g(s) 较低，而浇水良好的“Elvira”、“Florence”和“Cambridge Favourite”的 g(s) 相对较高。所有植物材料的 Delta C-13 在水分有限的情况下均高于浇水良好的植物，并且在品种之间表现出显着差异。在一项实验中，叶子 Delta C-13 表明“Elvira”的 WUE 最低，而“Totem”的 WUE 最高。 delta C-13 与热成像得出的 g(s) 指数呈负相关。尽管结果表明品种之间的 g(s) 和 WUE 存在显着差异，但一般来说，所有品种都通过降低 g(s) 从而增加 WUE 来应对水分亏缺。 (C) 2011 Elsevier B.V. 保留所有权利。
Cultivars with low stomatal conductance (g(s)) may show high water use efficiency (WUE) under drought conditions, but under optimal conditions low g(s) may result in low vigour. A combination of thermal imaging and carbon isotope composition (delta C-13) analysis offers potential for screening simultaneously for both high g(s) and high WUE. Ten cultivars of strawberry (Fragaria X ananassa Duch.) were grown in well watered or water limited conditions. Thermal images were taken of the plants, with various approaches to determine the optimal protocol for detecting variation in g(s), including use of reference leaves, grids to maintain leaves horizontal, and collection of meteorological data in synchrony with thermal images. delta C-13 of leaves, fruit, and crowns was determined. An index of g(s) derived from the temperature of horizontal leaves and the temperature of wet and dry references showed significant differences between cultivars and between well watered and water limited plants, as did g(s) estimated from leaf temperature, the temperature of a dry reference, and humidity. Thermal imaging indicated low g(s) in 'Elsanta' and 'Totem' and relatively high g(s) in well watered 'Elvira', 'Florence' and 'Cambridge Favourite'. delta C-13 of all plant material was higher in water limited than well watered plants and showed significant differences between cultivars. In one experiment leaf delta C-13 indicated lowest WUE in 'Elvira' and highest WUE in 'Totem'. delta C-13 was inversely correlated with an index of g(s) derived from thermal imaging. Although the results indicate substantial variation in g(s) and WUE between cultivars, generally all cultivars responded to water deficit by lowering g(s) and hence increasing WUE. (C) 2011 Elsevier B.V. All rights reserved.