Comparison of the effects of curcumin and curcumin glucuronide in human hepatocellular carcinoma HepG2 cells
Comparison of the effects of curcumin and curcumin glucuronide in human hepatocellular carcinoma HepG2 cells
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DOI：
10.1016/j.foodchem.2013.11.021
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发表时间：
2014-05-15
期刊：
影响因子：
8.8
通讯作者：
Miyazawa, Teruo
中科院分区：
文献类型：
--
作者：
Shoji, Motomu;Nakagawa, Kiyotaka;Miyazawa, Teruo
姜黄素是在姜黄（Curcuma Longa L）中发现的黄色色素，并且在最近的研究中报道具有几种药理作用，包括抗氧化、抗炎、抗肿瘤和降脂特性。然而，由于大多数姜黄素在通过肠道吸收时是结合的，因此游离姜黄素在体内的水平极低。因此，姜黄素代谢产物被认为是姜黄素生物活性的原因。在本研究中，我们首次证实了姜黄素葡萄糖醛酸苷是姜黄素在大鼠口服给药后血浆中的主要代谢产物。接下来，我们合成了姜黄素葡糖苷酸，并比较了姜黄素和姜黄素葡糖苷酸对人肝癌细胞系（HepG2）中基因表达的影响。我们发现姜黄素葡萄糖醛酸苷的作用弱于姜黄素，这种差异与姜黄素和姜黄素葡萄糖醛酸苷进入HepG2细胞的相对吸收率有关。(C)2013爱思唯尔有限公司保留所有权利。
Curcumin is a yellow pigment found in turmeric (Curcuma Longa L), and is reported, in recent studies, to have several pharmacological effects, including anti-oxidant, anti-inflammatory, anti-tumour and lipid-lowering properties. However, as most curcumin is conjugated when absorbed through the intestine, free curcumin is present at extremely low levels inside the body. Therefore, curcumin metabolites have been presumed to be responsible for the curcumin bioactivity. In this study, we first confirmed that curcumin glucuronide is the major metabolite of curcumin found in the plasma after oral administration of curcumin in rats. Next, we synthesised curcumin glucuronide and compared the effects of curcumin and curcumin glucuronide on gene expression in a human hepatoma cell line (HepG2). We found that the effects of curcumin glucuronide are weaker than those of curcumin and that this difference is related to relative absorption rates of curcumin and curcumin glucuronide into HepG2 cells. (C) 2013 Elsevier Ltd. All rights reserved.