Inhibition of female mating by male accessory gland substances in the ground beetle Leptocarabus procerulus
Inhibition of female mating by male accessory gland substances in the ground beetle Leptocarabus procerulus
复制标题
DOI：
10.1111/phen.12042
复制
发表时间：
2014-03-01
影响因子：
1.5
通讯作者：
Takami, Yasuoki
中科院分区：
文献类型：
--
作者：
Hayashi, Nao;Takami, Yasuoki
地甲Leptocarabus proerulus(Chaudoir)具有精液物质，具有形成交配塞子的物理功能和诱导雌性难治性行为的生理功能，这两种物质共同作用，阻碍雌性的繁殖。人们对导致女性难治性行为的物质的生理特性知之甚少，特别是关于它们的分泌器官、剂量依赖性、分子特征和对女性成熟度的影响。通过将男性衍生物质注射到女性体内，导致女性难治性行为的物质被证明是在男性的附腺中产生的，而不是在睾丸中产生的。注射附腺提取物在中等剂量时会增加雌性不应期，但在低剂量或高剂量时不会。相比之下，注射睾丸提取物可以在高剂量下缩短女性的不应期。高剂量的附腺物质缺乏效果的原因可能是直接注射的非自然大剂量精液产生的异常效应，阻止女性反应的精液物质的毒性，或者是促进女性接受能力的注射物质的反作用。副腺物质在受热时失去活性，尽管睾丸物质不会。没有成熟卵的雌性倾向于完全拒绝交配，尽管成熟卵数的变化(一个或多个)与雌性不育期无关，表明雌性生殖成熟的影响有限。这些发现可能有助于阐明精致雄性交配行为进化的生理基础。前遗症。
The ground beetleLeptocarabus procerulus(Chaudoir) possesses seminal substances that have a physical function to form mating plugs and a physiological function to induce female refractory behaviour, which act together to hinder female remating. Little is known about the physiological properties of the substances inducing female refractory behaviour, especially with respect to their secretory organ, dose‐dependency, molecular characteristics and the effect of female maturity. By injecting male‐derived substances into females, substances that induce female refractory behaviour are shown to be produced in the male accessory gland but not in the testis. Injection of extracts from the accessory gland increases the female refractory period at moderate doses but not at lower or higher doses. By contrast, injection of extracts from the testis reduces the female refractory period at high doses. The lack of an effect of accessory gland substances at higher doses could be the result of an anomalous effect of unnaturally large doses of seminal products by direct injection, the toxicity of seminal substances that deter female responses, or counteraction by injected substances that promote female receptivity. The accessory gland substances lose their activity when heated, although the testis substances do not. Females without mature eggs tend to reject mating entirely, although variation in the number of mature eggs (one or more) is not associated with the female refractory period, indicating the limited effect of female reproductive maturity. These findings may help to clarify the physiological basis of the evolution of the elaborated male mating behaviour inL. procerulus.