Hard x-ray phase contrast imaging of black lipid membranes
Hard x-ray phase contrast imaging of black lipid membranes
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DOI：
10.1063/1.3263946
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发表时间：
2009-11-16
影响因子：
4
通讯作者：
Salditt, T.
中科院分区：
文献类型：
--
作者：
Beerlink, A.;Mell, M.;Salditt, T.
我们报告硬X射线相衬成像的黑色脂质膜，自由悬浮在微机械孔径在水溶液中。因此，生物分子和有机物质可以探测在水合环境中的平行光束传播成像，使用相干多千电子伏的X射线辐射。薄膜的宽度可以从菲涅耳衍射区中的强度条纹的分析中分辨出来，低至约200 nm。的变薄过程中，其中溶剂从两个相对的单分子层之间的空间中排出，进行监测，和共存的溶胀和变薄的膜补丁的域之间的域壁的特征在于。
We report hard x-ray phase contrast imaging of black lipid membranes, freely suspended over a micromachined aperture in an aqueous solution. Biomolecular and organic substances can thus be probed in hydrated environments by parallel beam propagation imaging, using coherent multi-kilo-electronvolt x-ray radiation. The width of the thinning film can be resolved from analysis of the intensity fringes in the Fresnel diffraction regime down to about 200 nm. The thinning process, in which solvent is expelled from the space in between two opposing monolayers, is monitored, and the domain walls between coexisting domains of swollen and thinned membrane patches are characterized.