Control of alternative behavioral states by serotonin in Caenorhabditis elegans
Control of alternative behavioral states by serotonin in Caenorhabditis elegans
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DOI：
10.1016/s0896-6273(00)80527-9
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发表时间：
1998-07-01
期刊：
影响因子：
16.2
通讯作者：
Schafer, WR
中科院分区：
文献类型：
--
作者：
Waggoner, LE;Zhou, GT;Schafer, WR
血清素与多种脑功能的调节有关，包括对情绪、攻击性、性和睡眠的控制。在这里，我们报道了秀丽隐杆线虫，血清素控制着两种不同的，开/关状态的产卵行为之间的开关。通过对产蛋事件时间模式的定量分析，我们确定产蛋可以被建模为一个新的随机过程，在这个过程中，动物在离散的行为状态之间波动：活跃状态，在这个状态下，蛋会聚集在一起，而不活跃状态，在这个状态下，蛋会保留。单细胞消融术实验表明，HSNL/HSNR和VC4/VC5两对运动神经元可通过释放血清素诱导活跃期。这些神经元还会释放乙酰胆碱，这似乎会在活跃阶段触发单个的产卵事件。遗传实验表明，秀丽隐杆线虫产卵行为状态的决定可能是通过蛋白激酶c依赖（pkc依赖）对电压门控钙通道的调节介导的。
Serotonin has been implicated in the regulation of a wide range of brain functions involving alternative behavioral states, including the control of mood, aggression, sex, and sleep. Here, we report that in the nematode Caenorhabditis elegans, serotonin controls a switch between two distinct, on/off states of egg-laying behavior. Through quantitative analysis of the temporal pattern of egg-laying events, we determined that egg laying can be modeled as a novel random process, in which animals fluctuate between discrete behavioral states: an active state, during which eggs are laid in clusters, and an inactive state, during which eggs are retained. Single-cell ablation experiments indicate that two pairs of motor neurons, HSNL/HSNR and VC4/VC5, can induce the active phase by releasing serotonin. These neurons also release acetylcholine, which appears to trigger individual egg-laying events within the active phase. Genetic experiments suggest that determination of the behavioral states observed for C. elegans egg laying may be mediated through protein kinase C-dependent (PKC-dependent) modulation of voltage-gated calcium channels.