Effect of recombinant human bone morphogenetic protein-2/absorbable collagen sponge (rhBMP-2/ACS) on healing in 3-wall intrabony defects in dogs
Effect of recombinant human bone morphogenetic protein-2/absorbable collagen sponge (rhBMP-2/ACS) on healing in 3-wall intrabony defects in dogs
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DOI：
10.1902/jop.2002.73.1.63
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发表时间：
2002-01-01
影响因子：
4.3
通讯作者：
Wikesjö, UME
中科院分区：
文献类型：
--
作者：
Choi, SH;Kim, CK;Wikesjö, UME
背景资料：重组人骨形态发生蛋白-2（rhBMP-2）在可吸收胶原海绵（ACS）载体中被评估为牙周再生的候选疗法。本研究的目的是评估再生的牙槽骨和牙骨质，相关的根吸收和强直手术植入rhBMP-2/ACS在犬临床model.Methods：双边3壁骨内牙周缺损手术诱导在前磨牙区的上颌骨和下颌骨在8个年轻的成年韩国杂种狗。每只动物的缺损接受rhBMP-2/ACS（rhBMP-2浓度为0.2 mg/ml，总植入物体积/缺损类似于0.1 ml）或缓冲液/ACS，或作为假手术对照。对每只动物的survival进行测序，以提供8周和24周愈合间隔后的尸检观察结果。治疗结果进行了评价，使用临床，放射学和histometric parameters.Results：手术植入rhBMP-2/ACS导致加速增强骨形成的3壁骨内牙周缺损，但没有明显的增强牙骨质再生。rhBMP-2/ACS没有出现相关的异常愈合事件，如根吸收和强直在这些模拟的临床conditions.Conclusions：手术植入rhBMP-2/ACS可以安全地用于支持牙槽骨再生在犬骨内牙周缺损，没有异常事件，如根吸收或强直并发再生程序。rhBMP-2/ACS在该模型中似乎对牙骨质再生和功能性牙周韧带的形成没有显著影响。
Background: Recombinant human bone morphogenetic protein-2 (rhBMP-2) in an absorbable Collagen sponge (ACS) carrier is being evaluated as a candidate therapy for periodontal regeneration. The objective of this study was to evaluate regeneration of alveolar bone and cementum, and associated root resorption and ankylosis following surgical implantation of rhBMP-2/ACS in a canine clinical model.Methods: Bilateral 3-wall intrabony periodontal defects were surgically induced in the premolar region in the maxilla and mandible in 8 young adult Korean mongrel dogs. The defects in each animal received rhBMP-2/ACS (rhBMP-2 at 0.2 mg/ml, total implant volume/defect similar to 0.1 ml) or buffer/ACS, or served as sham-operated controls. Surgeries were sequenced for each animal to provide postmortem observations following 8- and 24-week healing intervals. Treatment outcomes were evaluated using clinical, radiographic, and histometric parameters.Results: Surgical implantation of rhBMP-2/ACS resulted in accelerated enhanced bone formation in the 3-wall intrabony periodontal defects but in no apparent enhancement of cementum regeneration. rhBMP-2/ACS did not appear to be associated with aberrant healing events such as root resorption and ankylosis under these simulated clinical conditions.Conclusions: Surgical implantation of rhBMP-2/ACS may be used safely to support regeneration of alveolar bone in intrabony periodontal defects in dogs without aberrant events such as root resorption or ankylosis complicating the regenerative procedure. rhBMP-2/ACS does not appear to have a significant effect on cementum regeneration and formation of a functional periodontal ligament in this model.