Human transformer-2-beta gene (SFRS10): Complete nucleotide sequence, chromosomal localization, and generation of a tissue-specific isoform
Human transformer-2-beta gene (SFRS10): Complete nucleotide sequence, chromosomal localization, and generation of a tissue-specific isoform
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DOI：
10.1006/geno.1998.5471
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发表时间：
1998-10-15
期刊：
影响因子：
4.4
通讯作者：
Stamm, S
中科院分区：
文献类型：
--
作者：
Nayler, O;Cap, C;Stamm, S
Htra2-beta是果蝇Transform-2的人类同源物，是SR样蛋白家族的成员。在这里，我们报告了完整的htra2-beta基因(HGMW批准的符号SFRS10)的分离和鉴定。该基因全长21,232个碱基，由10个外显子和9个内含子组成。辐射杂交定位将该基因定位在3q染色体上。转录起始密码子的上游区域包含一个Alu元件和几个潜在的转录因子结合位点。RT-PCR和与EST克隆的比较显示，通过选择性剪接产生了五种不同的RNA亚型。这些异构体编码三个不同的开放阅读框，其中两个htra2-beta3和htra2-beta4缺少第一个SR结构域。Htra2-beta3受发育调控，主要在脑、肝脏、睾丸中表达，在肾脏中表达较弱。此外，htra2-beta3的结构域结构类似于在果蝇雄性生殖系中发现的一个变体，这表明替代转换器-a变体具有显著的保守性。最后，我们证明htra2-beta3在细胞核中表达，并在酵母双杂交系统中和体内与SR蛋白的子集相互作用。(C)1998年学术出版社。
Htra2-beta is a human homologue of Drosophila transformer-2 and a member of the SR-like protein family. Here we report the isolation and characterization of the complete htra2-beta gene (HGMW-approved symbol SFRS10). The gene spans 21,232 bp and is composed of 10 exons and 9 introns. Radiation hybrid mapping localized the gene to chromosome 3q. The region upstream of the transcription initiation codon contains an Alu element and several potential transcription factor binding sites. RT-PCR and comparison with EST clones revealed five different RNA isoforms generated by alternative splicing. These isoforms encode three diverging open reading frames, and two of these, htra2-beta3 and htra2-beta4, lack the first SR domain. Htra2-beta3 is developmentally regulated and expressed predominantly in brain, liver testis, and weakly in kidney. Furthermore, the domain structure of htra2-beta3 resembles a variant found in the Drosophila male germline, indicating a remarkable conservation of alternative transformer-a variants. Finally, we show that htra2-beta3 is expressed in the nucleus and interacts with a subset of SR proteins in a yeast two-hybrid system and in vivo. (C) 1998 Academic Press.