Silencing of unpaired chromatin and histone H2A ubiquitination in mammalian meiosis
Silencing of unpaired chromatin and histone H2A ubiquitination in mammalian meiosis
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DOI：
10.1128/mcb.25.3.1041-1053.2005
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发表时间：
2005-02-01
影响因子：
5.3
通讯作者：
Grootegoed, JA
中科院分区：
文献类型：
--
作者：
Baarends, WM;Wassenaar, E;Grootegoed, JA
在雄性哺乳动物的减数分裂前期，X和Y染色体合并到XY体中。这种异染色质体是转录沉默的，并通过组蛋白H2A的泛素化增加来标记。这使我们更详细地研究组蛋白H2A泛素化和染色质沉默之间的关系。首先，我们发现泛素化的H2 A也标记女性体细胞中巴尔体的沉默X染色体。接下来，我们研究了H2A泛素化之间的可能关系。染色质沉默减数分裂前期有不成对的染色质。使用的小鼠模型在雄性减数分裂中携带不配对的常染色体区域，或在雌性减数分裂中携带不配对的X和Y染色体。我们发现泛素化组蛋白H2A与大染色质区域的转录沉默有关。哺乳动物减数分裂前期细胞中的这种沉默涉及未配对的染色质区域，类似于真菌粗糙脉孢菌中描述的现象，称为未配对DNA的减数分裂沉默。
During meiotic prophase in male mammals, the X and Y chromosomes are incorporated in the XY body. This heterochromatic body is transcriptionally silenced and marked by increased ubiquitination of histone H2A. This led us to investigate the relationship between histone H2A ubiquitination and chromatin silencing in more detail. First, we found that ubiquitinated H2A also marks the silenced X chromosome of the Barr body in female somatic cells. Next, we studied a possible relationship between H2A ubiquitination. chromatin silencing. and unpaired chromatin in meiotic prophase. The mouse models used carry an unpaired autosomal region in male meiosis or unpaired X and Y chromosomes in female meiosis. We show that ubiquitinated histone H2A is associated with transcriptional silencing of large chromatin regions. This silencing in mammalian meiotic prophase cells concerns unpaired chromatin regions and resembles a phenomenon described for the fungus Neurospora crassa and named meiotic silencing by unpaired DNA.