Macromolecular crowding impacts on the diffusion and conformation of DNA hairpins.
Macromolecular crowding impacts on the diffusion and conformation of DNA hairpins.
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DOI：
10.1103/physreve.91.012703
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发表时间：
2015-01
期刊：
影响因子：
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通讯作者：
Olivia Stiehl;Kathrin Weidner-Hertrampf;M. Weiss
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文献类型：
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作者：
Olivia Stiehl;Kathrin Weidner-Hertrampf;M. Weiss
拥挤流体中的生化反应与稀溶液中的生化反应有很大不同。这两个，排除体积与周围的大分子（“拥挤”）和增强的反应伙伴由于拥挤诱导的粘弹性和亚扩散的再结合的相互作用已被假设为转移化学平衡向相关的状态。我们已经探索了这两个线索在实验可调系统的影响，通过监测开放的DNA发夹在拥挤的流体具有不同的粘弹性特征，但类似的占用体积分数的稳态分数。结果，我们观察到粘弹性拥挤流体中闭合DNA发夹的比例增加。我们的观察结果相比，一个简单的统计模型，认为这两个方面的拥挤，而优惠之间的相互作用拥挤和DNA发夹似乎没有什么影响。
Biochemical reactions in crowded fluids differ significantly from those in dilute solutions. Both, excluded-volume interactions with surrounding macromolecules ("crowders") and an enhanced rebinding of reaction partners due to crowding-induced viscoelasticity and subdiffusion have been hypothesized to shift chemical equilibria towards the associated state. We have explored the impact of both cues in an experimentally tunable system by monitoring the steady-state fraction of open DNA hairpins in crowded fluids with varying viscoelastic characteristics but similar occupied volume fractions. As a result, we observed an increased fraction of closed DNA hairpins in viscoelastic crowded fluids. Our observations compare favorably to a simple statistical model that considers both facets of crowding, while preferential interactions between crowders and DNA hairpins appear to have little influence.