The genetic association study between polymorphisms in uncoupling protein 2 and uncoupling protein 3 and metabolic data in dogs.
The genetic association study between polymorphisms in uncoupling protein 2 and uncoupling protein 3 and metabolic data in dogs.
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DOI：
10.1186/1756-0500-7-904
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发表时间：
2014-12-11
影响因子：
1.8
通讯作者：
Omi T
中科院分区：
文献类型：
--
作者：
Udagawa C;Tada N;Asano J;Ishioka K;Ochiai K;Bonkobara M;Tsuchida S;Omi T
线粒体内膜解偶联蛋白（UCPs）是线粒体阴离子载体蛋白家族的成员，在能量平衡中起重要作用。遗传关联研究表明，人类UCP 2和UCP 3变体（SNP和插入缺失）与肥胖、胰岛素抵抗、2型糖尿病和代谢综合征相关。本研究的目的是检查UCP 2和UCP 3多态性与犬的代谢数据之间的遗传关联。通过对11个不同犬种（n = 119）的DNA序列分析，我们在UCP 2中鉴定出10个SNPs（9个内含子和1个外显子）和4个indel（内含子），在UCP 3中鉴定出13个SNPs（11个内含子和2个外显子）和1个indel（外显子）。这些UCP 2和UCP 3变异体和拉布拉多猎犬（n = 50）的葡萄糖，总胆固醇，乳酸脱氢酶和甘油三酯的生化参数之间的关联研究表明，没有UCP 2的多态性与这些参数的水平显着相关。然而，四个UCP 3 SNPs（内含子1）与总胆固醇水平显著相关。此外，与对照品种（Shiba，n = 30）相比，在对高胆固醇血症易感的品种（设得兰群岛牧羊犬，n = 30）中，四个SNP中的两个的等位基因频率与较高的总胆固醇水平相关。从有限数量的个体中获得的结果表明，狗中的UCP 3基因可能与总胆固醇水平相关。更大样本量的检查和进一步分析将提高这些结果的精确度。本文的在线版本（doi：10.1186/1756-0500-7-904）包含补充材料，可供授权用户使用。
The uncoupling proteins (UCPs) in the mitochondrial inner membrane are members of the mitochondrial anion carrier protein family that play an important role in energy homeostasis. Genetic association studies have shown that human UCP2 and UCP3 variants (SNPs and indels) are associated with obesity, insulin resistance, type 2 diabetes mellitus, and metabolic syndrome. The aim of this study was to examine the genetic association between polymorphisms in UCP2 and UCP3 and metabolic data in dogs. We identified 10 SNPs (9 intronic and 1 exonic) and 4 indels (intronic) in UCP2, and 13 SNPs (11 intronic and 2 exonic) and one indel (exonic) in UCP3, by DNA sequence analysis of 11 different dog breeds (n = 119). An association study between these UCP2 and UCP3 variants and the biochemical parameters of glucose, total cholesterol, lactate dehydrogenase and triglyceride in Labrador Retrievers (n = 50) showed that none of the UCP2 polymorphisms were significantly associated with the levels of these parameters. However, four UCP3 SNPs (intron 1) were significantly associated with total cholesterol levels. In addition, the allele frequencies of two of the four SNPs associated with higher total cholesterol levels in a breed that is susceptible to hypercholesterolemia (Shetland Sheepdogs, n = 30), compared with the control breed (Shiba, n = 30). The results obtained from a limited number of individuals suggest that the UCP3 gene in dogs may be associated with total cholesterol levels. The examination of larger sample sizes and further analysis will lead to increased precision of these results. The online version of this article (doi:10.1186/1756-0500-7-904) contains supplementary material, which is available to authorized users.