Germ cell specific expression of Vasa in rare minnow, Gobiocypris rarus
Germ cell specific expression of Vasa in rare minnow, Gobiocypris rarus
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Vasa 在稀有米诺鱼（稀有鲫鱼）生殖细胞中的特异性表达
DOI：
10.1016/j.cbpa.2012.02.007
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发表时间：
2012-07-01
影响因子：
2.3
通讯作者：
Zhao, Haobin
中科院分区：
文献类型：
--
作者：
Cao, Mengxi;Yang, Yuhui;Zhao, Haobin
生殖细胞很早就与体细胞一起被搁置，并在物种的繁衍过程中发挥作用，从一代到下一代。瓦萨是死亡家族的一员，在动物界被认为是生殖细胞标记物。稀有金枪鱼是中国研究发育和毒理学等方面的新兴模式鱼，选择合适的生殖细胞标记将有助于研究可能影响生殖细胞发育的因素。在这里，我们报道了具有Vasa蛋白典型特征的稀有海绵Grvas的克隆和鉴定。RT-PCR结果表明，Grvas在雄性和雌性的性腺中特异表达，母体沉积到卵子中用于胚胎，并在从受精卵到幼虫和成虫的胚胎中持续表达。Grvas基因和/或蛋白仅局限于卵巢和睾丸的生殖细胞。Grvas基因在胚胎发育过程中的时间表达与斑马鱼血管的表达相似。GRAVS信号与原始生殖细胞一致。这些结果意味着生殖细胞标记Grvas是从稀有的小鱼中分离出来的，并且它只在生殖细胞中表达。(C)2012 Elsevier Inc.保留所有权利。
Germ cells are set aside early with somatic cells and take roles for reproduction of species from one generation to the next generation. Vasa, a member of DEAD family is well documented as germ cell marker in the animal kingdom. Rare minnow, Gobiocypris rarus, is an emerging model fish in China to study development and toxicology, etc. A suitable germ cell marker will benefit the studies of the factors that may influence germ cell development. Here, we report the cloning and characterization of G. rarus vasa named Grvas whose protein product has the typical characteristics of Vasa proteins. RT-PCR results showed that Grvas is expressed specifically in the gonads of male and female, it is maternally deposited into the eggs for embryos and is continuously expressed in the embryos from the zygote to larvae and adult. Grvas mRNA and/or protein is restricted to the germ cells of ovary and testis. Temporal expression of Grvas mRNA is similar to that of zebrafish vasa during embryogenesis. Grvas signals are coincident with primordial germ cells. These result; mean that a germ cell marker, Grvas is isolated from rare minnow and its expression is exclusively in germ cells. (C) 2012 Elsevier Inc. All rights reserved.