Effect of hypothermia on motor function of adult rats after neonatal hyperthermic hypoxic-ischemic brain insult
Effect of hypothermia on motor function of adult rats after neonatal hyperthermic hypoxic-ischemic brain insult
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DOI：
10.1007/s00421-009-1156-9
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发表时间：
2010-05-01
影响因子：
3
通讯作者：
Iseki, Takumi
中科院分区：
文献类型：
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作者：
Hosono, Takayoshi;Kamo, Ayumi;Iseki, Takumi
在低温治疗新生儿高温性缺氧缺血性脑病(HIE)方面，我们研究了新生高温性HIE大鼠模型的运动功能，并利用该模型进行了全身低温治疗。本研究选用新生7日龄Wistar大鼠42只。34只新生大鼠在异氟醚麻醉下结扎左侧颈总动脉。另设假手术组(S组，n=8)。恢复1h后，所有大鼠在40A℃的环境温度(T(A))下暴露于8%氧气15min。损伤后，16只大鼠(H组)和18只大鼠(N组)在动脉结扎后分别置于30A(A)和37A(A)的舱室中(N组)，S组所有大鼠在37A(A)℃舱中放置12h，8周龄所有大鼠进行Rota-Rod测试。棒分别以5、5和7rpm的速度旋转，连续三天。仅在测量第二天，N组大鼠在转杆上停留的时间明显短于S组和H组。N组大鼠损伤侧大脑半球宽度明显小于S组和H组。在运动功能和解剖学方面，S组和H组之间无显著相关性。这些结果提示，新生儿高温性缺氧缺血性损伤可损害运动功能，损伤后全身降温可恢复运动功能。
Regarding therapeutic hypothermia for human neonatal hyperthermic hypoxic-ischemic encephalopathy (HIE), we investigated the motor function of a neonatal hyperthermic HIE rat model, and also performed systemic hypothermia using the model. Forty-two neonatal Wistar rats at 7-days-old were used in this study. The left common carotid artery of 34 neonatal rats was ligated under isoflurane anesthesia. We also established a sham group (S group, n = 8). After 1-h recovery, all rats were exposed to 8% oxygen at an ambient temperature (T (a)) of 40A degrees C for 15 min. Following insult, 16 rats were placed in a chamber at a T (a) of 30A degrees C (H group) and the other 18 rats at a T (a) of 37A degrees C after arterial ligation (N group), and all rats in the S group were placed in a chamber at a T (a) of 37A degrees C for 12 h. A Rota-Rod test was performed involving all rats at 8 weeks old. The rod was rotated at 5, 5, and 7 rpm on three consecutive days, respectively. Rats in the N group stayed on the rotating rod for a significantly shorter period than those in S and H groups only on the second day of measurement. The width of the insulted hemisphere in N group rats was significantly smaller than those in S and H groups. There was no significant correlation between S and H groups regarding the motor function and anatomy. These results suggest that neonatal hyperthermic hypoxic-ischemic insult impairs the motor function, which may be rescued by systemic hypothermia after insult.