Intraprostatic capsaicin injection as a novel model for nonbacterial prostatitis and effects of botulinum toxin A
Intraprostatic capsaicin injection as a novel model for nonbacterial prostatitis and effects of botulinum toxin A
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DOI：
10.1016/j.eururo.2006.11.037
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发表时间：
2007-04-01
期刊：
影响因子：
23.4
通讯作者：
Chancellor, Michael B.
中科院分区：
文献类型：
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作者：
Chuang, Yao-Chi;Yoshimura, Naoki;Chancellor, Michael B.
目的：用前列腺内注射辣椒素建立大鼠非细菌性前列腺炎动物模型，辣椒素被认为能刺激c -传入纤维并引起神经源性炎症。A型肉毒毒素（BoNT-A）的镇痛和抗炎性能在该模型中进行了测试。方法：将不同剂量的辣椒素注入成年雄性spraquedawley大鼠前列腺。使用行为法评估辣椒素的伤害效应30分钟；然后切除前列腺进行组织学检查和环氧化酶（COX） 2蛋白浓度测定。静脉注射埃文斯蓝（50mg /kg）以评估血浆蛋白外渗。第二组动物在前列腺内注射1000 μ mol/l辣椒素1周之前，在前列腺内注射高达20 U的BoNT-A。结果：辣椒素剂量依赖性诱导疼痛行为改变：闭眼、低运动、炎症改变：急性期炎症细胞积聚、COX2表达、血浆外渗增加，1周后完全恢复。BoNT-A预处理剂量依赖性地逆转疼痛行为和炎症。BoNT-A 20 U显著降低炎症细胞积聚、COX2表达和埃文斯蓝提取（分别为82.1%、83.0%和50.4%），减少疼痛行为（视力评分66.7%，运动评分46.5%）。结论：前列腺内注射辣椒素可引起神经源性前列腺炎和前列腺疼痛，可能是一种有益的研究模型。BoNT-A具有抗炎和镇痛作用，支持前列腺炎的临床评价。(c) 2006年欧洲泌尿外科协会。Elsevier B.V.版权所有。
Objectives: An animal model for nonbacterial prostatitis in rats was developed with the use of intraprostatic injection of capsaicin, an agent thought to excite C-afferent fibers and cause neurogenic inflammation. The analgesic and antiinflammatory properties of botulinum toxin type A (BoNT-A) was tested in this model.Methods: Adult male Spraque-Dawley rats were injected with varying doses of capsaicin into the prostate. The nociceptive effects of capsaicin were evaluated for 30 min by using a behavior approach; then the prostate was removed for histology and cyclooxygenase (COX) 2 protein coneentration measurement. Evans blue (50 mg/kg) was also injected intravenously to assess for plasma protein extravasation. A second set of animals were injected with up to 20 U of BoNT-A into the prostates 1 wk prior to intraprostatic injection of 1000 mu mol/l capsaicin.Results: Capsaicin dose dependently induced modifications in pain behavior: closing of the eyes, hypolocomotion, and inflammatory changes: increase of inflammatory cell accumulation, COX2 expression, and plasma extravasation at the acute stage, but completely recovered at 1 wk. BoNT-A pretreatment dose dependently reversed pain behavior and inflammation. BoNT-A 20 U significantly decreased inflammatory cell accumulation, COX2 expression, and Evans blue extraction (82.1%, 83.0%, and 50.4%, respectively), and reduced pain behavior (66.7% for eye score and 46.5% for locomotion score).Conclusions: Intraprostatic capsaicin injection induced neurogenic prostatitis and prostatic pain, and may be a useful research model. BoNT-A produced anti-inflammatory and analgesic effects, and support clinical evaluation in prostatitis. (c) 2006 European Association of Urology. Published by Elsevier B.V. All rights reserved.