Cyclic AMP regulates the proportion of functional acetylcholine receptors on chicken ciliary ganglion neurons.
Cyclic AMP regulates the proportion of functional acetylcholine receptors on chicken ciliary ganglion neurons.
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环 AMP 调节鸡睫状神经节神经元上功能性乙酰胆碱受体的比例。
DOI：
10.1073/pnas.84.22.8155
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发表时间：
1987
影响因子：
11.1
通讯作者：
Dionne,VE
中科院分区：
文献类型：
--
作者：
Margiotta,JF;Berg,DK;Dionne,VE
先前的研究表明，培养物中鸡睫状神经节神经元上功能性乙酰胆碱受体（AcChoR）的数量远小于标记受体探针在神经元上检测到的 AcChoR 总数。在这里，我们使用膜片钳记录来表明 cAMP 依赖性过程通过可能涉及功能性 AcChoR 数量增加的机制来增强神经元的 AcCho 反应。这种增加不需要蛋白质合成，也不涉及用标记受体探针测量的细胞表面上 AcChoR 总数的可检测增加。结果表明，神经元具有功能性和非功能性 AcChoR 库，并且可以通过 cAMP 依赖性过程从细胞内受体或细胞表面非功能性受体招募功能性受体。对功能性神经递质受体数量的 cAMP 依赖性调节将提供一种可逆机制，通过该机制，细胞间相互作用可以调节神经系统中的突触传递。
Previous studies have shown that the number of functional acetylcholine receptors (AcChoRs) on chicken ciliary ganglion neurons in culture is considerably smaller than the total number of AcChoRs detected on the neurons by labeled receptor probes. Here we use patch-clamp recording to show that a cAMP-dependent process enhances the AcCho response of the neurons by a mechanism likely to involve an increase in the number of functional AcChoRs. The increase occurs without requiring protein synthesis and without involving a detectable increase in the total number of AcChoRs on the cell surface measured with a labeled receptor probe. The results imply that the neurons have functional and nonfunctional pools of AcChoRs and that functional receptors can be recruited from intracellular receptors or from nonfunctional receptors on the cell surface by a cAMP-dependent process. A cAMP-dependent regulation of the number of functional neurotransmitter receptors would provide a reversible mechanism by which cell-cell interactions could modulate synaptic transmission in the nervous system.