A method for measuring multiple cytokines from small samples
A method for measuring multiple cytokines from small samples
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DOI：
10.1016/j.bbi.2003.09.009
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发表时间：
2004-05-01
影响因子：
15.1
通讯作者：
Watkins, LR
中科院分区：
文献类型：
--
作者：
O'Connor, KA;Holguin, A;Watkins, LR
Commercially available enzyme-linked immunosorbent assay (ELISA) kits are commonly used to assess levels of proinflammatory cytokines in biological samples. Most of these kits require sample volumes of at least 50 mul. Thus, in order to examine multiple cytokines, volumes greater than 100 mul must be collected. However, the volume of many biological samples, especially those collected from the central nervous system (i.e., microdialysates, push-pull perfusions, or cerebrospinal fluid samples), is much less than 100 mul. Therefore, we developed a method for analyzing multiple cytokines from a single, low-volume biological sample, which involves serially assaying the samples on multiple proinflammatory cytokine ELISA kits. In many cases, assaying for one cytokine does not interfere with subsequent assay for another cytokine in the same sample. Moreover, when interference is observed, the interfering factor can be identified and its effect minimized. (C) 2004 Elsevier Inc. All rights reserved.
