Newly delivered transferrin iron and oxidative cell injury
Newly delivered transferrin iron and oxidative cell injury
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DOI：
10.1016/s0014-5793(97)00056-2
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发表时间：
1997-02-17
期刊：
影响因子：
3.5
通讯作者：
Cabantchik, ZI
中科院分区：
文献类型：
--
作者：
Breuer, W;Greenberg, E;Cabantchik, ZI
细胞铁状态的评估是根据其介导细胞损伤的能力由促氧化剂。培养的K562细胞在短暂暴露于转铁蛋白(Tf)和叔丁基氢过氧化氢(TBHP)后，发生了明显的变化：(A)可检测到的LIP升高，(B)增加了脂质过氧化反应，(C)最终细胞死亡：所有这些影响都被弱碱或铁络合剂抑制，类似地，过氧化氢引起LIP和2‘，7’-二氯荧光素二醋酸酯可检测到的氧化剂种类的增加，而与TF预先孵育可增强这一变化。Tf传递的铁从LIP和TBHP反应池中消失，t(1/2)<；30min。结果表明，铁在内体和胞浆配体之间的转运过程中催化潜力最大。(C)1997年欧洲生化学会联合会。
Cell iron status was assessed in terms of its capacity to mediate cell injury by pro-oxidants. Cultured K562 cells, which maintain a stable cytosolic labile iron pool (LIP) of < 0.5 mu M, underwent distinct changes after short exposures to transferrin (Tf) followed by t-butyl hydroperoxide (TBHP): (a) rise in LIP, detectable fluorimetrically (b) increased lipid peroxidation and (c) eventual cell death: All of these effects were inhibited by weak bases or iron chelators, Similarly, hydrogen peroxide caused rises in both LIP and oxidant species detectable with 2',7'-dichlorofluorescin diacetate, which were enhanced by preincubation with Tf. The Tf-delivered iron disappeared from LIP and the TBHP-reactive pool with a t(1/2) < 30 min. The results indicate that the catalytic potential of iron is highest while in transit between endosomes and cytosolic ligands. (C) 1997 Federation of European Biochemical Societies.