Hypomethylation of intron1 of α-synuclein gene does not correlate with Parkinson's disease.
Hypomethylation of intron1 of α-synuclein gene does not correlate with Parkinson's disease.
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DOI：
10.1186/s13041-017-0285-z
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发表时间：
2017-02-07
期刊：
影响因子：
3.6
通讯作者：
Kim YS
中科院分区：
文献类型：
--
作者：
Guhathakurta S;Evangelista BA;Ghosh S;Basu S;Kim YS
Deregulation of α-synuclein encoding gene (SNCA) is one of the important facets of Parkinson’s disease (PD) research. DNA methylation status of SNCA-intron1 has been shown to regulate the α-synuclein expression. The present study is aimed at investigating whether methylation of SNCA-intron1 is associated with higher expression of α-synuclein in PD. We have investigated the intron1 methylation status from 16 post-mortem brain samples comprised of 8 PD and 8 control subjects using bisulfite sequencing. We further correlated this methylation status with α-synuclein protein levels in substantia nigra of that individual using western blot analysis. We did not observe any significant difference in methylation of SNCA-intron1 region between PD and control samples. Moreover, no correlation was observed between methylation of SNCA-intron1 with α-synuclein level. Methylation of SNCA-intron1 region does not correlate with α-synuclein expression in PD samples.
