Photoactivatable carbene-generating phospholipids: physical properties and use in detection of phase separations in lipid mixtures.
Photoactivatable carbene-generating phospholipids: physical properties and use in detection of phase separations in lipid mixtures.
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光活化卡宾生成磷脂：物理性质及其在脂质混合物相分离检测中的应用。
DOI：
10.1021/bi00508a052
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发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
Khorana,HG
中科院分区：
文献类型：
--
作者：
Curatolo,W;Radhakrishnan,R;Gupta,CM;Khorana,HG
摘要：携带光反应性卡宾生成基团的磷脂已与多种受体磷脂在二元和三元混合物中发生了交联反应，并且结果与所涉及的脂类的物理行为相关联。用差示扫描量热法研究了光反应磷脂1-PAL-MITO-2-[W-NIZ‘-重氮-S’，3‘，3’-三氟丙氧基]-磷脂酰胆碱(PCI)和1-棕榈酰基-2-[-(间-重氮基-诺苯氧基)十一酰基]磷脂酰胆碱(PCII)分别在~17℃和~6℃发生特征的酰链有序-无序转变。在这些脂类与二棕榈酰卵磷脂(DPL)的等摩尔混合物中，相分离发生在特征温度以下，正如以前在饱和和不饱和磷脂混合物中观察到的那样。饱和和非饱和条件下PCIi或PCII混合物的光解
William Curatolo, 1 Ramachandran Radhakrishnan, Chhitar M. Gupta, s and H. Gobind Khorana** abstract: Phospholipids which carry photoreactive carbene-generating groups havebeen cross-linked with a variety of acceptor phospholipids in binary and ternary mixtures, and the results have been correlated with the physical behavior of the lipids involved. The photoreactive phospholipids 1-pal-mitoyl-2-[w-ÍZ'-diazo-S', 3', 3'-trifluoropropionoxy) lauroyl]-phosphatidylcholine (PCI) and 1-palmitoyl-2-[-(m-diaziri-nophenoxy) undecanoyl] phosphatidylcholine (PCII) undergo characteristic acyl chain order-disorder transitions at~ 17 and~ 6 C, respectively, as demonstrated by differential scanning calorimetry. In equimolar mixtures of these lipids with dipalmitoyllecithin (DPL), phase separation occurs below characteristic temperatures as has been previously observed for mixtures of saturated and unsaturated phospholipids. Photolysis of mixtures of PCI or PCII with saturated or un-