Functional implications of the Klenow fragment structure.
Functional implications of the Klenow fragment structure.
复制标题
克列诺片段结构的功能意义。
DOI：
10.1042/bst0140205
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发表时间：
1986
影响因子：
3.9
通讯作者：
Grindley,JM
中科院分区：
文献类型：
--
作者：
Steitz,TA;Freemont,PS;Ollis,DL;Joyce,CM;Grindley,JM
在DNA聚合酶的研究中有三个特别感兴趣的问题。首先，这种酶在确保模板指导的DNA合成的保真度方面起着什么作用？酶如何减少不正确碱基的掺入并检测和去除错配碱基对？第二，是什么解释了酶的持续合成能力，即连续掺入核苷酸而不发生酶的解离？第三，我们如何想象生物前聚合酶的进化？大肠杆菌DNA聚合酶I首先由Kornberg及其同事分离，并且已成为理解DNA聚合酶如何工作的最合适的酶（Kornberg，1980）。Pol 1是一个分子量为103 000道尔顿的单体，具有DNA聚合酶、3 '-5'核酸外切酶和5 '-3'核酸外切酶活性，并具有单独的脱氧核苷单磷酸和三磷酸结合位点
There are three issues of particular interest in studies of DNA polymerases. First, what role does the enzyme play in assuring the fidelity of template-directed DNA synthesis? How does the enzyme reduce the incorporation of incorrect bases and detect and remove mismatch base-pairs? Second, what accounts for the enzyme's processivity, ie the successive incorporation of nucleotides without enzyme dissociation? Third, how might we imagine that prebiotic polymerases evolved? Escherichia coli DNA polymerase I was first isolated by Kornberg and colleagues and has become a most suitable enzyme for understanding how DNA polymerases work (Kornberg, 1980). Pol1 is a 103 000 dalton monomer, has DNA polymerase, 3'-5'exonuclease and 5'-3'exonuclease activity and has separate binding sites for deoxynucleoside monophosphate and triphosphate