Identification and Comparative Quantification of Bio-Active Phthalides in Essential Oils from Si-Wu-Tang, Fo-Shou-San, Radix Angelica and Rhizoma Chuanxiong
Identification and Comparative Quantification of Bio-Active Phthalides in Essential Oils from Si-Wu-Tang, Fo-Shou-San, Radix Angelica and Rhizoma Chuanxiong
复制标题
四物汤、佛手散、当归和川芎精油中生物活性苯酞的鉴定和比较定量
DOI：
10.3390/molecules15010341
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发表时间：
2010-01-01
期刊：
影响因子：
4.6
通讯作者：
Ding, Anwei
中科院分区：
文献类型：
--
作者：
Tang, Yuping;Zhu, Min;Ding, Anwei
酞类化合物是以当归、白芷为主要原料的四物汤和佛手散两种常用中药复方中的重要生物活性成分。本文采用气相色谱-质谱联用（GC-MS）法测定或估算了8种苯酞类化合物的含量，包括Z-藁本内酯、E-藁本内酯、Z-丁烯基苯酞、E-丁烯基苯酞、3-丁基苯酞、新烟碱和川芎素A。结果表明，GC-MS法是一种简便、快速、灵敏度高的分析四物汤、佛手散、当归、川芎中苯酞类化合物的方法，当归、川芎配伍成四物汤、佛手散等复方制剂后，Z-藁本内酯、E-藁本内酯、Z-丁烯苯酞等苯酞类化合物的可提取量发生变化。体外子宫收缩抑制实验结果表明，四物汤、佛手散、当归、川芎挥发油的活性随苯酞类化合物含量的增加而增强，说明苯酞类化合物可能是四物汤、佛手散、当归、川芎挥发油抑制小鼠子宫收缩的主要活性成分。研究结果表明，对各味中药及方剂的化学成分和药理活性进行比较分析，有助于阐明中药的活性成分，揭示中药方剂的配伍机理。
Phthalides are important bio-active constituents in Si-Wu-Tang and Fo-Shou-San, two commonly used Traditional Chinese Medicine (TCM) combined prescriptions mainly derived from Radix Angelica and Rhizoma Chuanxiong. In this paper, the contents of eight phthalides, including Z-ligustilide, E-ligustilide, Z-butylenephthalide, E-butylene-phthalide, 3-butylphthalide, neocnidilide and senkyunolide A were determined or estimated by gas chromatography-mass spectrometry (GC-MS). The results showed GC-MS was a simple, rapid, and high sensitive method for analyzing phthalides in Si-Wu-Tang, Fo-Shou-San, Radix Angelica and Rhizoma Chuanxiong, and the extractable contents of each phthalides including Z-ligustilide, E-ligustilide, Z-butylenephthalide, etc. varied after Radix Angelica, Rhizoma Chuanxiong were combined into a formulation, such as Si-Wu-Tang and Fo-Shou-San. Furthermore, inhibition activity of essential oils from Si-Wu-Tang, Fo-Shou-San, Radix Angelica and Rhizoma Chuanxiong on uterine contraction was tested in an in vitro assay, and the results showed that the activity of the essential oil is higher as the content of the phthalides increase, which demonstrated that phthalides are possibly main active components inhibiting mice uterine contraction in vitro. All of the results suggested that comparative analysis of chemical components and pharmacological activities of each herb and formula is possibly helpful to elucidate the active components in traditional Chinese medicine, and to reveal the compatibility mechanism of TCM formulae.