Proteomics computational analyses suggest that the carboxyl terminal glycoproteins of Bunyaviruses are class II viral fusion protein (beta-penetrenes).
Proteomics computational analyses suggest that the carboxyl terminal glycoproteins of Bunyaviruses are class II viral fusion protein (beta-penetrenes).
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DOI：
10.1186/1742-4682-1-10
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发表时间：
2004-11-15
影响因子：
--
通讯作者：
Garry RF
中科院分区：
文献类型：
--
作者：
Garry CE;Garry RF
布尼亚病毒科(Bunyaviridae)囊膜RNA病毒家族包括五个属：正病毒、汉坦病毒、静脉病毒、奈罗病毒和冠状病毒。目前还不能确定是哪种布尼亚病毒蛋白介导了病毒粒子：细胞膜融合。由甲病毒科和黄病毒科成员编码的第II类病毒融合蛋白(β-穿透素)由3个反平行的β-折叠结构域组成，在第II结构域的末端有一个内部融合肽。蛋白质组学计算表明，由细小病毒沙蝇热病毒(SAN)编码的羧基末端糖蛋白(GC)与甲型病毒辛德比斯病毒(SIN)的II类融合蛋白包膜蛋白1(E1)具有显著的氨基酸序列相似性。相似的序列和共同的结构/功能基序，包括高倾向于与双层膜结合的结构域，共线位于San GC和Sin E1中。由每个布尼亚病毒属的成员编码的GC具有几个序列和结构基序。这些结果表明，布尼亚病毒科的GC以及Tenui病毒和一组秀丽线虫逆转录病毒的相似蛋白是II类病毒融合蛋白。对不同的病毒融合蛋白的比较可以揭示病毒粒子的基本特征：细胞融合，并建议药物和疫苗策略。
The Bunyaviridae family of enveloped RNA viruses includes five genuses, orthobunyaviruses, hantaviruses, phleboviruses, nairoviruses and tospoviruses. It has not been determined which Bunyavirus protein mediates virion:cell membrane fusion. Class II viral fusion proteins (beta-penetrenes), encoded by members of the Alphaviridae and Flaviviridae, are comprised of three antiparallel beta sheet domains with an internal fusion peptide located at the end of domain II. Proteomics computational analyses indicate that the carboxyl terminal glycoprotein (Gc) encoded by Sandfly fever virus (SAN), a phlebovirus, has a significant amino acid sequence similarity with envelope protein 1 (E1), the class II fusion protein of Sindbis virus (SIN), an Alphavirus. Similar sequences and common structural/functional motifs, including domains with a high propensity to interface with bilayer membranes, are located collinearly in SAN Gc and SIN E1. Gc encoded by members of each Bunyavirus genus share several sequence and structural motifs. These results suggest that Gc of Bunyaviridae, and similar proteins of Tenuiviruses and a group of Caenorhabditis elegans retroviruses, are class II viral fusion proteins. Comparisons of divergent viral fusion proteins can reveal features essential for virion:cell fusion, and suggest drug and vaccine strategies.