Change in the transfer profile of orally administered tetrodotoxin to non-toxic cultured pufferfish Takifugu rubripes depending of its development stage
Change in the transfer profile of orally administered tetrodotoxin to non-toxic cultured pufferfish Takifugu rubripes depending of its development stage
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DOI：
10.1016/j.toxicon.2013.01.011
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发表时间：
2013-04-01
期刊：
影响因子：
2.8
通讯作者：
Arakawa, Osamu
中科院分区：
文献类型：
--
作者：
Tatsuno, Ryohei;Shikina, Miwako;Arakawa, Osamu
为了研究生长（器官发育）对河豚鱼体内河豚毒素（TTX）动态的影响，将含TTX的饲料匀浆以40小鼠单位（MU）（8.8 μ g）/20 g体重的剂量经口灌胃给予6月龄和15月龄的红鳍东方鲀无毒培养样本。24 h后，处死标本，分离皮肤组织（背侧和腹侧）、肌肉、肝脏、消化道和性腺。TTX含量（微克/克），在每个组织中，通过液相色谱/质谱法测定，揭示了TTX的分布概况，特别是TTX含量的肝脏，两个年龄之间有很大的差异; TTX得分的15个月大的鱼（33微克/克）是近5倍的6个月大的鱼（0.68微克/克）。在6个月龄的鱼中，个体的总剩余TTX量（相对于给定剂量的量）为31%，其中71%在皮肤中，在15个月龄的鱼中为84%，其中83%在肝脏中。性腺指数（GSI）和肝指数（HSI）的分数，性腺和肝脏的组织学观察表明，虽然这两个年龄之间的成熟阶段差异不大，有明确的区别，在肝脏的发育阶段。结果表明，TTX在T.红足与肝脏的发育有关，即，通过食物生物体进入河豚体内的TTX被消除或主要转移到具有未发育肝脏的幼鱼的皮肤上，但是随着鱼的生长和肝脏的继续发育，大部分TTX被转移并积累在肝脏中。(C)2013爱思唯尔有限公司保留所有权利。
To investigate the effects of growth (organ development) on tetrodotoxin (TTX) dynamics in the pufferfish body, TTX-containing feed homogenate was administered to 6- and 15-month old non-toxic cultured specimens of the pufferfish Takifugu rubripes at a dose of 40 mouse units (MU) (8.8 mu g)/20 g body weight by oral gavage. After 24 h, the specimens were killed and the skin tissues (dorsal and ventral), muscle, liver, digestive tract, and gonads were separated. TTX content (mu g/g) in each tissue, determined by liquid chromatography/mass spectrometry, revealed that the TTX distribution profile, particularly the TTX content of the liver, greatly differed between the two ages; the TTX score of 15-month old fish (33 mu g/g) was nearly 5-fold that of 6-month old fish (0.68 mu g/g). The total remaining TTX amount per individual (relative amount to the given dose) was 31% in 6-month old fish, of which 71% was in the skin, and 84% in 15-month old fish, of which 83% was in the liver. The gonadosomatic index (GSI) and hepatosomatic index (HSI) scores, and histologic observations of the gonads and liver suggest that although there is little difference in maturation stage between these two ages, there are clear distinctions in the developmental stage of the liver. The results suggest that the TTX dynamics in T. rubripes are linked to the development of the liver, i.e., the TTX taken up into the pufferfish body via food organisms is eliminated or transferred mainly to the skin in young fish with an undeveloped liver, but as the fish grow and the liver continues to develop, most of the TTX is transferred to and accumulated in the liver. (C) 2013 Elsevier Ltd. All rights reserved.