Differential Expression of Serum Proteins in Rats with Allergic Asthma: A Study Based on the Nanoliter Two-Dimensional Liquid Chromatography Technique.
Differential Expression of Serum Proteins in Rats with Allergic Asthma: A Study Based on the Nanoliter Two-Dimensional Liquid Chromatography Technique.
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DOI：
10.1155/2017/8015703
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发表时间：
2017
影响因子：
2.2
通讯作者：
Cui H
中科院分区：
文献类型：
--
作者：
Yan X;Zhang A;Yu L;Chen C;Cui H
Objective.目的：应用纳升二维液相色谱（nano-2D-LC）技术研究过敏性哮喘的发病机制。法24只Wistar大鼠随机分为空白对照组（A组）、束缚组（B）和哮喘模型组（C组）。采用纳升二维液相色谱技术研究哮喘大鼠血清蛋白质的差异表达。结果与束缚组相比，哮喘模型组的数据显示了一个独特的峰，即峰数13（94.731 min）。数据还显示哮喘组中有三个缺失的峰，即峰号1（77.489 min）、峰号2（78.418 min）和峰号5（80.533 min），表明相应的肽可能与哮喘的发病机制有关。与空白对照组相比，抑制组出现4个新峰，即峰数2（78.418 min）、峰数4（79.398 min）、峰数5（80.533 min）和峰数7（81.824 min）。束缚组也出现了一个缺失的峰，即峰数3（78.804 min），表明这5种多肽可能与束缚诱导的应激刺激有关。结论本研究提示过敏性哮喘的发病机制与蛋白质水平异常密切相关，但未来的动物实验应明确应激因素引起的特异性蛋白质表达。
Objective. To investigate the pathogenesis of allergic asthma via the nanoliter two-dimensional liquid chromatography (nano-2D-LC) technique. Method. 24 Wistar rats were randomized into 3 groups: the blank-control group (A), the restrained group (B), and the asthma model group (C). The nanoliter two-dimensional liquid chromatography (nano-2D-LC) technique was used to study the differential protein expressions of the serum in asthmatic rats. Results. Compared with the restrained group, the data from the asthma model group displayed a distinctive peak, that is, peak number 13 (94.731 min). The data also displayed three missing peaks in the asthma group, that is, peak number 1 (77.489 min), peak number 2 (78.418 min), and peak number 5 (80.533 min), suggesting that the corresponding peptides might be related to the pathogenesis of asthma. Compared with the blank-control group, the restrained group showed 4 new peaks, that is, peak number 2 (78.418 min), peak number 4 (79.398 min), peak number 5 (80.533 min), and peak number 7 (81.824 min). The restrained group also displayed a missing peak, that is, peak number 3 (78.804 min), indicating that those 5 polypeptides might be related to the binding-induced stress stimuli. Conclusion. The study suggests that the pathogenesis of allergic asthma is closely related to abnormal levels of proteins; however, future animal experiments should identify the specific protein expressions caused by stress factors.