Localization of antigens associated with adherens junctions, desmosomes, and hemidesmosomes during murine molar morphogenesis
Localization of antigens associated with adherens junctions, desmosomes, and hemidesmosomes during murine molar morphogenesis
复制标题
DOI：
10.1046/j.1432-0436.1998.6310001.x
复制
发表时间：
1998-05-01
期刊：
影响因子：
2.9
通讯作者：
Lesot, H
中科院分区：
文献类型：
--
作者：
Fausser, JL;Schlepp, O;Lesot, H
上皮间质相互作用在牙形成过程中起着至关重要的作用。由于上皮细胞-细胞和细胞-基质的相互作用也可能参与牙釉质器官的组织形态发生，我们利用间接免疫荧光研究了小鼠和大鼠第一下颌磨牙中连接复合物相关蛋白的定位。使用针对E-cadherin、β -catenin和血小板红蛋白（γ -catenin）的抗体检测粘附连接。桥粒用抗桥粒蛋白抗体定位，半桥粒用抗BP-230和HD-1蛋白抗体定位。当内牙上皮分化时，可见e -钙粘蛋白、血小板红蛋白和BP-230的减少。牙外侧和内侧之间存在不对称分布的斑红蛋白、粘粒蛋白和BP-230；粘连蛋白最初局限于管状网齿，逐渐在星状网、中间层和成釉细胞基极中积累。这些抗原的特定时空分布模式提示粘附连接、桥粒和半桥粒直接参与小鼠第一下磨牙的发育。
Epitheliomesenchymal interactions are known to play a crucial role during odontogenesis. Since epithelial cell-cell and cell-matrix interactions may also be involved in enamel organ histomorphogenesis, we investigated the localization of proteins associated with junctional complexes in mouse and rat first lower molars by indirect immunofluorescence. Adherens junctions were detected using antibodies directed against E-cadherin, beta-catenin, and plakoglobin (gamma-catenin). Desmosomes were localized with antibodies against desmoglein, and hemidesmosomes using antibodies against BP-230 and HD-1 proteins. When the inner dental epithelium differentiates, a decrease of E-cadherin, plakoglobin, and BP-230 is seen. An asymmetric distribution of plakoglobin, desmoglein, and BP-230 between the lateral and medial side of the tooth exists; desmoglein, which was first restricted to the gubernaculum dentis, progressively accumulated in the stellate reticulum, the stratum intermedium, and the basal pole of ameloblasts. The specific temporospatial distributions patterns of these antigens suggests a direct involvement of adherens junctions, desmosomes, and hemidesmosomes in the development of the murine first lower molar.