Oncogenicity of friend‐virus‐infected cells: Determination of origin of spleen colonies by the H‐2 antigens as genetic markers
Oncogenicity of friend‐virus‐infected cells: Determination of origin of spleen colonies by the H‐2 antigens as genetic markers
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友病毒感染细胞的致癌性：以 H-2 抗原作为遗传标记确定脾集落的起源
DOI：
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发表时间：
1978
影响因子：
6.4
通讯作者：
P. Tambourin
中科院分区：
文献类型：
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作者：
F. Wendling;P. Tambourin
经静脉途径接种Friend白血病病毒(FLV)的小鼠的脾细胞在正常F1组织相容的杂交宿主的脾中产生肉眼可见的集落。用H-2抗原作为鉴定脾细胞来源菌株的通用标记进行了研究。该标准细胞毒试验适用于鉴定正常或白血病亲本或F1杂交系小鼠脾细胞表面的H-2抗原。(C3H×C57BL/6)F1受者(H-2k/H-2b)静脉移植C3H来源的脾细胞(H-2k)10天后，从脾中分离出的单个克隆对抗C57BL/6抗体均敏感。同样，用DBA/2白血病脾细胞(H-2d)移植的(DBA/2×C57BL/10)F1受者(H-2d/H-2b)的脾细胞集落对抗H-2b和抗H-2d抗体均敏感。这些结果直接证明了构成脾细胞集落的主要细胞群来自受体。作者认为，脾细胞集落不是由于注射的供体细胞的肿瘤增殖所致，而是由移植细胞产生的Friend病毒转化的宿主细胞的增殖所致。
Spleen cells from mice infected with Friend leukemia virus (FLV) inoculated by the intravenous route give rise to macroscopically visible colonies in the spleens of normal F1 histocompatible hybrid hosts. A study of H‐2 antigens as generic markers for identification of strains of origin of cells constituting the spleen colonies was undertaken. The standard cytotoxic test was demonstrated to be suitable for characterizing the H‐2 antigens present on the surface of spleen cells from normal or FLV‐leukemic parents or F1 hybrid mice. Individual colonies dissected out of the spleen of (C3H × C57BL/6) F1 recipients (H‐2k/H‐2b), 10 days after the intravenous graft of FLV‐infected spleen cells of C3H origin (H‐2k), were all sensitive to anti‐C57BL/6 antibodies. In the same way, colonies obtained from the spleens of (DBA/2 × C57BL/10) F1 recipients (H‐2d/H‐2b) grafted with DBA/2 leukemic spleen cells (H‐2d) were all sensitive to both anti‐H‐2b and anti‐H‐2d antibodies. These results directly prove that the main cell population constituing a spleen colony arises from the recipient. The authors conclude that the spleen colonies do not result from the neoplastic proliferation of injected donor cells but rather from the multiplication of host cells transformed by Friend virus produced by the grafted cells.