A detailed linkage map of rainbow trout produced using doubled haploids.
A detailed linkage map of rainbow trout produced using doubled haploids.
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使用双单倍体产生的虹鳟鱼的详细连锁图。
DOI：
10.1093/genetics/148.2.839
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发表时间：
1998
期刊：
影响因子：
3.3
通讯作者：
Thorgaard,GH
中科院分区：
文献类型：
--
作者：
Young,WP;Wheeler,PA;Coryell,VH;Keim,P;Thorgaard,GH
我们报道了虹鳟鱼（Oncorhynchus mykiss）的第一个详细的遗传连锁图谱。以“OSU”和“Arlee”雄性纯合系杂交获得的76条双单倍体虹鳟鱼为材料，进行了分离分析。476个标记被划分为31个大连锁群和11个小连锁群（<5个/组）。最小基因组长度估计为2627.5 cM。性别决定位点分离到一个连锁群的远端位置。我们分析了三类标记的染色体分布：(1)扩增片段长度多态性，(2)串联重复数可变，以及(3)利用与鲑鱼特异性小分散核元件5 ‘或3 ’端同源的探针获得的标记。许多第一类标记聚集在似乎与着丝粒相对应的区域。第二类标记的端粒分布偏多，第三类标记的端粒分布偏中间。四体遗传，显然与鲑科鱼类的四倍体祖先有关，在一个简单的序列重复位点上被检测到，并通过一个极大的连锁组的存在提出，该连锁组似乎由两个在其尖端相连的较小的组组成。双单倍体虹鳟鱼系和连锁图谱为进一步的基因组研究奠定了基础。
We report the first detailed genetic linkage map of rainbow trout (Oncorhynchus mykiss). The segregation analysis was performed using 76 doubled haploid rainbow trout produced by androgenesis from a hybrid between the “OSU” and “Arlee” androgenetically derived homozygous lines. Four hundred and seventy-six markers segregated into 31 major linkage groups and 11 small groups (<5 markers/group). The minimum genome size is estimated to be 2627.5 cM in length. The sex-determining locus segregated to a distal position on one of the linkage groups. We analyzed the chromosomal distribution of three classes of markers: (1) amplified fragment length polymorphisms, (2) variable number of tandem repeats, and (3) markers obtained using probes homologous to the 5′ or 3′ end of salmonid-specific small interspersed nuclear elements. Many of the first class of markers were clustered in regions that appear to correspond to centromeres. The second class of markers were more telomeric in distribution, and the third class were intermediate. Tetrasomic inheritance, apparently related to the tetraploid ancestry of salmonid fishes, was detected at one simple sequence repeat locus and suggested by the presence of one extremely large linkage group that appeared to consist of two smaller groups linked at their tips. The double haploid rainbow trout lines and linkage map present a foundation for further genomic studies.