BIOCHEMICAL MODELING OF AN AUTONOMOUSLY OSCILLATORY CIRCADIAN CLOCK IN EUGLENA
BIOCHEMICAL MODELING OF AN AUTONOMOUSLY OSCILLATORY CIRCADIAN CLOCK IN EUGLENA
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DOI：
10.1126/science.2988128
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发表时间：
1985-01-01
期刊：
影响因子：
56.9
通讯作者：
EDMUNDS, LN
中科院分区：
文献类型：
--
作者：
GOTO, K;LAVALMARTIN, DL;EDMUNDS, LN
真核微生物，以及高等动植物，表现出许多自主的生理和生化节律，周期约为24小时。为了确定导致这些昼夜节律周期的计时机制的性质，我们假设了两个主要的操作假设。生物钟的假定元素在其正常振荡范围内的扰动，以及对这种元素的直接激活和抑制，都应该产生由潜在振荡器产生的显性节律的相移。在鞭毛虫中进行的实验结果表明，烟酰胺腺嘌呤二核苷酸(NAD+)、线粒体钙转运系统、钙离子、钙调蛋白、NAD+激酶和NADP+磷酸酶代表时钟“齿轮”，在这种生物和其他生物中，可能构成一个自我维持的昼夜振荡循环。
Eukaryotic microorganisms, as well as higher animals and plants, display many autonomous physiological and biochemical rhythmicities having periods approximating 24 hours. In an attempt to determine the nature of the timing mechanisms that are responsible for these circadian periodicities, two primary operational assumptions were postulated. Both the perturbation of a putative element of a circadian clock within its normal oscillatory range and the direct activation as well as the inhibition of such an element should yield a phase shift of an overt rhythm generated by the underlying oscillator. Results of experiments conducted in the flagellate Euglena suggest that nicotinamide adenine dinucleotide (NAD+), the mitochondrial Ca2+-transport system, Ca2+, calmodulin, NAD+kinase, and NADP+phosphatase represent clock "gears" that, in ensemble, might constitute a self-sustained circadian oscillating loop in this and other organisms.