Regulation of Acyl Homoserine Lactone Synthesis in Pseudomonas putida JMQS1 Under Phenol Stress
Regulation of Acyl Homoserine Lactone Synthesis in Pseudomonas putida JMQS1 Under Phenol Stress
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DOI：
10.1007/s11270-016-3018-5
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发表时间：
2016-09-01
影响因子：
2.9
通讯作者：
Jayachandran, K.
中科院分区：
文献类型：
--
作者：
Antony, Merlin;Jayachandran, K.
革兰氏阴性细菌中细胞密度依赖性基因表达是通过酰基高丝氨酸内酯信号分子的活性实现的。从受洗涤剂污染的土壤中分离出的新菌株恶臭假单胞菌 JMQS1 表现出群体感应以及降解苯酚的能力。使用对紫色色杆菌 DSTS-1 突变生物传感器和 luxI 和 luxR 基因特异性扩增的响应来表征分离株的群体感应特性。通过液相色谱-四极杆飞行时间可以鉴定各种酰基高丝氨酸内酯分子的合成调节，即苯酚应激初始阶段的C6HSL、降解期间的C8HSL以及降解完成时的3OC(12)HSL。薄层色谱、傅里叶变换红外光谱和气相色谱质谱证实苯酚在48-56小时内完全降解。 P. putida JMQS1 通过根据不断变化的生理条件选择性激活群体感应信号分子来表现出对苯酚胁迫的适应。本研究提出了一种通过补充酰基高丝氨酸内酯信号分子来增强有毒有机污染物降解的有效方法。
The cell density-dependent gene expression in gram-negative bacteria is through the activity of acyl homoserine lactone signal molecules. The novel strain Pseudomonas putida JMQS1 isolated from detergent-contaminated soil exhibited quorum sensing along with its ability to degrade phenol. The response to Chromobacterium violaceum DSTS-1 mutant biosensor and luxI and luxR gene-specific amplification was used to characterize the quorum sensing property of the isolate. A regulation in the synthesis of various acyl homoserine lactone molecules, viz C6HSL in the initial stages of phenol stress, C8HSL during degradation, and 3OC(12)HSL on completion of degradation could be identified by liquid chromatography-quadrupole time of flight. Thin-layer chromatography, Fourier transform infrared spectroscopy, and gas chromatography mass spectrometry confirmed the complete degradation of phenol in 48-56 h. P. putida JMQS1 exhibited adaptation over phenol stress through the selective activation of the quorum sensing signal molecules depending on the changing physiological conditions. This study proposes an efficient method for enhancing the degradation of toxic organic pollutants by the supplementation of acyl homoserine lactone signal mole cules.