Proteomics analysis of the peritoneal dialysate effluent reveals the presence of calcium-regulation proteins and acute inflammatory response
Proteomics analysis of the peritoneal dialysate effluent reveals the presence of calcium-regulation proteins and acute inflammatory response
复制标题
DOI：
10.1186/1559-0275-11-17
复制
发表时间：
2014-01-01
影响因子：
3.8
通讯作者：
Santos, Hugo M.
中科院分区：
文献类型：
--
作者：
Oliveira, Elisabete;Araujo, Jose E.;Santos, Hugo M.
背景：腹膜透析（PD）是一种用于晚期慢性肾脏疾病的肾脏替代形式。PD流出物具有用于诊断和预后的生物标志物发现的巨大潜力。在这项研究中，一种新的方法来解开蛋白质组的基础上二硫苏糖醇耗尽，其次是2D-SDS-PAGE和蛋白质鉴定，使用串联massspectrometry proposed.Results：共分析了49个点，揭示了25个差异表达的蛋白质，其中许多蛋白质参与钙调节。值得注意的是，已经发现一组处理钙代谢和钙调节的蛋白质通过腹膜透析液流出物丢失，从而给出了腹膜透析和肾损伤患者软组织钙化的可能解释。由于样本处理和分析方法的差异，很难比较处理PD的文献。
Background: Peritoneal dialysis (PD) is a form of renal replacement used for advanced chronic kidney disease. PD effluent holds a great potential for biomarker discovery for diagnosis and prognosis. In this study a novel approach to unravelling the proteome of PD effluent based-on dithiothreitol depletion followed by 2D-SDS-PAGE and protein identification using tandem mass spectrometry is proposed.Results: A total of 49 spots were analysed revealing 25 proteins differentially expressed, among them many proteins involved in calcium regulation.Conclusions: Remarkably, a group of proteins dealing with calcium metabolism and calcium regulation has been found to be lost through peritoneal dialysate effluent, giving thus a potential explanation to the calcification of soft tissues in patients subjected to peritoneal dialysis and kidney injury. Comparison of literature dealing with PD is difficult due to differences in sample treatment and analytical methodologies.