A FRAMESHIFT MUTATION PREVENTS KUNITZ TRYPSIN-INHIBITOR MESSENGER-RNA ACCUMULATION IN SOYBEAN EMBRYOS
A FRAMESHIFT MUTATION PREVENTS KUNITZ TRYPSIN-INHIBITOR MESSENGER-RNA ACCUMULATION IN SOYBEAN EMBRYOS
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DOI：
10.1105/tpc.1.4.427
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发表时间：
1989-04-01
期刊：
影响因子：
11.6
通讯作者：
GOLDBERG, RB
中科院分区：
文献类型：
--
作者：
JOFUKU, KD;SCHIPPER, RD;GOLDBERG, RB
我们研究了大豆Kunitz胰蛋白酶抑制剂（KTi）基因突变的分子基础，该基因突变阻止了种子发育过程中Kunitz胰蛋白酶抑制剂蛋白的积累。我们发现，编码主要Kunitz胰蛋白酶抑制剂蛋白（KTi 3 mRNA）的mRNA在空（KTi-）胚胎中减少至少100倍，但KTi 3基因转录活性在Kunitz胰蛋白酶抑制剂产生胚胎（KTi+）和KTi-胚胎中相似。我们对来自KTi 3+和KTi 3-系的Kunitz胰蛋白酶抑制剂KTi 3基因进行了测序，发现这些基因在KTi 3编码区内仅相差3个核苷酸（+481、+486和+487）。这些核苷酸的改变导致KT 13-基因内的移码，其导致翻译期间的提前终止。我们的研究结果表明，KTi 3-移码突变导致KTi 3- mRNA不稳定，并导致KTi 3 mRNA患病率急剧下降。
We investigated the molecular basis of a soybean Kunitz trypsin inhibitor (KTi) gene mutation that prevents the accumulation of Kunitz trypsin inhibitor protein during seed development. We found that mRNA encoding the major Kunitz trypsin inhibitor protein (KTi3 mRNA) is reduced at least 100-fold in null (KTi-) embryos but that KTi3 gene transcriptional activity is similar in Kunitz trypsin inhibitor producing embryos (KTi+) and in KTi- embryos. We sequenced the Kunitz trypsin inhibitor KTi3 gene from both KTi3+ and KTi3- lines and found that these genes differ by only three nucleotides (+481, +486, and +487) within the KTi3 coding region. Alteration of these nucleotides results in a frameshift within the KTi3- gene that causes premature termination during translation. Our results suggest that the KTi3- frameshift mutation results in KTi3- mRNA destabilization and leads to a drastic reduction in KTi3 mRNA prevalence.