Programming Cells for Dynamic Assembly of Inorganic Nano-Objects with Spatiotemporal Control
Programming Cells for Dynamic Assembly of Inorganic Nano-Objects with Spatiotemporal Control
复制标题
时空控制动态组装无机纳米物体的编程单元
DOI：
10.1002/adma.201705968
复制
发表时间：
2018
影响因子：
29.4
通讯作者：
Zhong Chao
中科院分区：
文献类型：
--
作者：
Wang Xinyu;Pu Jiahua;An Bolin;Li Yingfeng;Shang Yuequn;Ning Zhijun;Liu Yi;Ba Fang;Zhang Jiaming;Zhong Chao
编程活细胞以时空精确的方式组织无机纳米物体（NOs）将推进新技术，用于创建有序的NOs和具有新兴功能的新生物-非生物混合材料。细菌细胞通常生长在称为生物膜的细胞群落中。在这里，报告了一种用于编程动态生物膜形成的策略，用于以动态、可扩展和分层的方式在不同界面上同步组装离散的NO或异质纳米结构。通过改造大肠杆菌感受蓝光并通过产生生物膜卷曲纤维来响应，生物膜的形成在空间上受到控制，同时实现了图案化的NO的组装。展示了最小图案化分辨率为100 µm的多样化和复杂的荧光量子点图案。通过在时间上控制NO向培养物中的顺序添加，通过自主的逐层组装来制造多层异质结构薄膜。它表明，生物动态自组装可用于推进一个新的剧目的纳米技术和材料的复杂性越来越大，否则将具有挑战性的生产。
Programming living cells to organize inorganic nano‐objects (NOs) in a spatiotemporally precise fashion would advance new techniques for creating ordered ensembles of NOs and new bio–abiotic hybrid materials with emerging functionalities. Bacterial cells often grow in cellular communities called biofilms. Here, a strategy is reported for programming dynamic biofilm formation for the synchronized assembly of discrete NOs or hetero‐nanostructures on diverse interfaces in a dynamic, scalable, and hierarchical fashion. By engineeringEscherichia colito sense blue light and respond by producing biofilm curli fibers, biofilm formation is spatially controlled and the patterned NOs' assembly is simultaneously achieved. Diverse and complex fluorescent quantum dot patterns with a minimum patterning resolution of 100 µm are demonstrated. By temporally controlling the sequential addition of NOs into the culture, multilayered heterostructured thin films are fabricated through autonomous layer‐by‐layer assembly. It is demonstrated that biologically dynamic self‐assembly can be used to advance a new repertoire of nanotechnologies and materials with increasing complexity that would be otherwise challenging to produce.