Semipurified Fractions from the Submerged-Culture Broth of Agaricus blazei Murill Reduce Blood Glucose Levels in Streptozotocin-Induced Diabetic Rats
Semipurified Fractions from the Submerged-Culture Broth of Agaricus blazei Murill Reduce Blood Glucose Levels in Streptozotocin-Induced Diabetic Rats
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DOI：
10.1021/jf9036672
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发表时间：
2010-04-14
影响因子：
6.1
通讯作者：
Ha, Yeong L.
中科院分区：
文献类型：
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作者：
Oh, Tae W.;Kim, Yun A.;Ha, Yeong L.
在链脲佐菌素（60 mg/kg，腹腔内）诱导的糖尿病雄性Sprague道利大鼠中，相对于糖尿病药物二甲双胍，检查了来自巴西蘑菇深层培养液热水提取物的半纯化级分的降血糖作用。将热水提取物用乙醇处理以除去β-葡聚糖和糖蛋白，冷冻干燥，并分馏成己烷、氯仿、乙酸乙酯（EA）和丁醇级分。EA组分（EAF; 200 mg/kg体重）在口服葡萄糖耐量试验中降低了（p < 0.05）血糖水平。在一项为期14天的治疗研究中，用EAF治疗的糖尿病大鼠显示血糖水平受到抑制，血浆胰岛素和葡萄糖转运蛋白-4升高;与对照动物相比，这些反应以剂量依赖性方式（200和400 mg/kg体重）发生。EAF可降低血浆甘油三酯和胆固醇水平，降低血液中谷草转氨酶和谷丙转氨酶活性，降低肝、肾中硫代巴比妥酸反应物含量。EAF（400 mg/kg体重）的降血糖疗效与二甲双胍（500 mg/ kg体重）相似。EAF含有大量的黄酮类化合物，包括染料木黄酮、染料木苷、大豆苷元和大豆苷，这可能有助于该馏分的降血糖作用。这些结果表明，巴西蘑菇深层培养液的热水提取物含有具有有效降血糖作用的EAF，其可用于治疗糖尿病。
Hypoglycemic action of semipurified fractions from hot-water extracts of the submerged-culture broth of Agaricus blazei Murill was examined in streptozotocin (60 mg/kg, intraperitoneal)-induced diabetic male Sprague Dawley rats, relative to the diabetes drug metformin. The hot-water extract, treated with ethanol to remove beta-glucans and glycoproteins, was freeze-dried, and fractionated into hexane, chloroform, ethyl acetate (EA), and butanol fractions. The EA fraction (EAF; 200 mg/kg body weight) reduced (p < 0.05) the blood glucose level in the oral glucose tolerance test, relative to the other fractions and control. In a 14 day-treatment study, diabetic rats treated with the EAF displayed a suppressed blood glucose level and elevated plasma insulin and glucose transport-4 proteins; the reactions occurred in a dose-dependent manner (200 and 400 mg/kg body weight) compared to those in control animals. The EAF reduced the levels of triglyceride and cholesterol in plasma, the activity of glutamate oxaloacetate transaminase and glutamate-pyruvate transaminase in blood, and the content of thiobarbituric acid reactive substance in the liver and kidney. The hypoglycemic efficacy of the EAF (400 mg/kg body weight) was similar to that of metformin (500 mg/ kg body weight). The EAF contained substantial amounts of isoflavonoids including genistein, genistin, daidzein, and daidzin, which could have contributed to the fraction's hypoglycemic action. These results indicate that the hot-water extract of the submerged-culture broth of Agaricus blazei contains an EAF having potent hypoglycemic action, which could be useful in the treatment of diabetes mellitus.