Stabilization of dhurrin biosynthetic enzymes from Sorghum bicolor using a natural deep eutectic solvent
Stabilization of dhurrin biosynthetic enzymes from Sorghum bicolor using a natural deep eutectic solvent
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DOI：
10.1016/j.phytochem.2019.112214
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发表时间：
2020-02-01
期刊：
影响因子：
3.8
通讯作者：
Laursen, Tomas
中科院分区：
文献类型：
--
作者：
Knudsen, Camilla;Bavishi, Krutika;Laursen, Tomas
近年来，离子液体和低共熔溶剂（DES）由于其萃取和增溶代谢物和生物聚合物的能力远远超过其在油和水中的溶解度而受到越来越多的关注。天然存在的代谢物能够形成天然低共熔溶剂（NADES），从而构成除了水相和脂质相之外的第三个细胞内相的假设，促使研究人员研究NADES在生命系统中的作用。作为特殊代谢产物的优良溶剂，植物细胞脱水后形成的NADES可以为干旱期间酶的功能储存提供合适的环境。使用的酶催化的防御化合物dhurrin的生物合成的实验模型系统，我们表明，参与这一途径的酶表现出增加的稳定性NADES相比，水缓冲溶液，和酶的活性恢复后再水化。受大自然的启发，NADES的应用为长期储存包括膜锚定酶在内的整个生物合成途径提供了一种生物技术方法。
In recent years, ionic liquids and deep eutectic solvents (DESs) have gained increasing attention due to their ability to extract and solubilize metabolites and biopolymers in quantities far beyond their solubility in oil and water. The hypothesis that naturally occurring metabolites are able to form a natural deep eutectic solvent (NADES), thereby constituting a third intracellular phase in addition to the aqueous and lipid phases, has prompted researchers to study the role of NADES in living systems. As an excellent solvent for specialized metabolites, formation of NADES in response to dehydration of plant cells could provide an appropriate environment for the functional storage of enzymes during drought. Using the enzymes catalyzing the biosynthesis of the defense compound dhurrin as an experimental model system, we demonstrate that enzymes involved in this pathway exhibit increased stability in NADES compared with aqueous buffer solutions, and that enzyme activity is restored upon rehydration. Inspired by nature, application of NADES provides a biotechnological approach for long-term storage of entire biosynthetic pathways including membrane-anchored enzymes.