Construction of a map-based reference genome sequence for barley, Hordeum vulgare L.
Construction of a map-based reference genome sequence for barley, Hordeum vulgare L.
复制标题
DOI：
10.1038/sdata.2017.44
复制
发表时间：
2017-04-27
期刊：
影响因子：
9.8
通讯作者：
Mascher M
中科院分区：
文献类型：
--
作者：
Beier S;Himmelbach A;Colmsee C;Zhang XQ;Barrero RA;Zhang Q;Li L;Bayer M;Bolser D;Taudien S;Groth M;Felder M;Hastie A;Šimková H;Staňková H;Vrána J;Chan S;Muñoz-Amatriaín M;Ounit R;Wanamaker S;Schmutzer T;Aliyeva-Schnorr L;Grasso S;Tanskanen J;Sampath D;Heavens D;Cao S;Chapman B;Dai F;Han Y;Li H;Li X;Lin C;McCooke JK;Tan C;Wang S;Yin S;Zhou G;Poland JA;Bellgard MI;Houben A;Doležel J;Ayling S;Lonardi S;Langridge P;Muehlbauer GJ;Kersey P;Clark MD;Caccamo M;Schulman AH;Platzer M;Close TJ;Hansson M;Zhang G;Braumann I;Li C;Waugh R;Scholz U;Stein N;Mascher M
大麦（Hordeum vulgare L.）是一种禾本科牧草，主要用作动物饲料和制麦工业的原料。大麦基因组图位参考序列。“Morex”由国际大麦基因组测序联盟（IBSC）使用分层鸟枪测序构建。在这里，我们报告了实验和计算程序，以（i）测序和组装超过80，000个细菌人工染色体（BAC）克隆，沿着基因组范围物理图谱的最小平铺路径，（ii）发现和验证相邻BAC之间的重叠，（iii）构建4，265个非冗余序列支架，代表重叠BAC的簇，和（iv）使用由密集遗传图谱、光学图谱和染色体构象捕获测序（Hi-C）提供的位置信息，将这些BAC簇沿着七条大麦染色体排序和定向。大麦基因组探索者（BARLEX）提供了对这些序列和作图资源的综合访问。
Barley (Hordeum vulgare L.) is a cereal grass mainly used as animal fodder and raw material for the malting industry. The map-based reference genome sequence of barley cv. ‘Morex’ was constructed by the International Barley Genome Sequencing Consortium (IBSC) using hierarchical shotgun sequencing. Here, we report the experimental and computational procedures to (i) sequence and assemble more than 80,000 bacterial artificial chromosome (BAC) clones along the minimum tiling path of a genome-wide physical map, (ii) find and validate overlaps between adjacent BACs, (iii) construct 4,265 non-redundant sequence scaffolds representing clusters of overlapping BACs, and (iv) order and orient these BAC clusters along the seven barley chromosomes using positional information provided by dense genetic maps, an optical map and chromosome conformation capture sequencing (Hi-C). Integrative access to these sequence and mapping resources is provided by the barley genome explorer (BARLEX).
影响因子：
3.7
作者：
Asan;Geng C;Chen Y;Wu K;Cai Q;Wang Y;Lang Y;Cao H;Yang H;Wang J;Zhang X
通讯作者：
Zhang X
影响因子：
1.8
作者：
Taudien S;Steuernagel B;Ariyadasa R;Schulte D;Schmutzer T;Groth M;Felder M;Petzold A;Scholz U;Mayer KF;Stein N;Platzer M
通讯作者：
Platzer M