Roles of BCCIP in chromosome stability and cytokinesis
Roles of BCCIP in chromosome stability and cytokinesis
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DOI：
10.1038/sj.onc.1210460
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发表时间：
2007-09-20
期刊：
影响因子：
8
通讯作者：
Shen, Z.
中科院分区：
文献类型：
--
作者：
Meng, X.;Fan, J.;Shen, Z.
BRCA2基因参与重组DNA修复和胞质分裂。BRCA2缺陷与染色体异常有关，这是导致肿瘤发生的基因组不稳定的标志。在这里，我们发现在HT1080细胞中BRCA2相互作用蛋白(BCCIP)的下调导致染色体多倍化、中心体扩增和异常的有丝分裂纺锤体形成。BCCIP基因敲除的细胞可以进入有丝分裂并保留纺锤体检查点，但不能完成胞质分裂。我们的数据表明，BCCIP在维持基因组完整性方面发挥了重要作用。
The BRCA2 gene is involved in recombinational DNA repair and cytokinesis. BRCA2 defects are associated with chromosomal abnormalities, which is a hallmark of genomic instability that contributes to tumorigenesis. Here, we show that downregulation of a BRCA2 interacting protein ( BCCIP) in HT1080 cells leads to chromosomal polyploidization, centrosome amplification and abnormal mitotic spindle formation. The BCCIP knockdown cells can enter mitosis and retain spindle checkpoint, but fail to complete cytokinesis. Our data suggest an essential role of BCCIP in the maintenance of genomic integrity.