Osteogenic differentiation of human mesenchymal stem cells on poly(ethylene glycol)-variant biomaterials
Osteogenic differentiation of human mesenchymal stem cells on poly(ethylene glycol)-variant biomaterials
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DOI：
10.1002/jbm.a.32310
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发表时间：
2009-12-15
影响因子：
4.9
通讯作者：
Arinzeh, Treena Livingston
中科院分区：
文献类型：
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作者：
Briggs, Tonye;Treiser, Matthew D.;Arinzeh, Treena Livingston
本研究评估了人间充质干细胞 (MSCS) 在与聚乙二醇 (PEG) 共聚的酪氨酸衍生聚碳酸酯上的成骨分化，以确定其作为骨组织工程应用支架的潜力。在聚碳酸酯主链中添加 PEG 已被证明可以改变机械性能、降解速率、蛋白质吸附程度以及随后的细胞粘附和成熟细胞表型的运动性。它对 MSC 行为的影响尚不清楚。在含有 0-5% PEG 的聚碳酸酯上培养 14 天，评估 MSC 形态、运动性、增殖和成骨分化。碱性磷酸酶活性和骨钙素表达表明，尽管在 14 天培养的不同阶段，含有 0% 或 3% PEG 含量的聚碳酸酯上的 MSC 上调了成骨标志物的表达。不含 PEG 的聚碳酸酯上的细胞具有早期开始细胞扩散和成骨分化的特征。 3% PEG 表面上的细胞在细胞铺展和成骨分化方面延迟，但与早期时间点不含 PEG 的基质和含有 5% PEG 的基质上的细胞相比，具有最高的运动性。在整个培养过程中，含有 5% PEG 的聚碳酸酯上的细胞具有最低水平的成骨标记，表现出较差的细胞-基质粘附性，并建立了细胞-细胞聚集体。因此，设计具有 PEG 微小变化的基质可以作为指导 MSC 粘附和运动伴随成骨分化的工具，并且可能有利于体内丰富的骨、组织形成。 (C) 2008 Wiley periodicals, Inc. J Biomed Mater Res 91A: 975-984, 2009
This study evaluated the osteogenic differentiation of human mesenchymal stem cells (MSCS), on tyrosine-derived polycarbonates copolymerized with poly(ethylene glycol) (PEG) to determine their potential as a scaffold for bone tissue engineering applications. The addition of PEG in the backbone of polycarbonates has been shown to alter mechanical properties, degradation rates, degree of protein adsorption, and subsequent cell adhesion and motility in mature cell phenotypes. Its effect on MSC behavior is unknoxn. MSC morphology, motility, proliferation, and osteogenic differentiation were evaluated on polycarbonates containing 0-5% PEG over a 14 day culture. MSCs on polycarbonates containing 0% or 3%, PEG content upregulated the expression of osteogenic markers as demonstrated by alkaline phosphatase activity and osteocalcin expression although at different stages in the 14 day culture. Cells on polycarbonates containing no PEG were characterized as having early onset of cell spreading and osteogenic differentiation. Cells on 3% PEG surfaces were delayed in cell spreading and osteogenic differentiation, but had the highest motility as compared with cells on substrates containing no PEG and substrates containing 5% PEG at early time points. Throughout the Culture, cells on polycarbonates containing 5% PEG had the lowest levels of osteogenic markers, displayed poor cell-substrate adhesion, and established cell-cell aggregates. Thus, designing substrates with Minute variations in PEG may serve as a tool to guide MSC adhesion and motility accompanying osteogenic differentiation, and may be beneficial for abundant bone, tissue formation in vivo. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 91A: 975-984, 2009