It's Electric: When Technology Gives a Boost to Stem Cell Science
It's Electric: When Technology Gives a Boost to Stem Cell Science
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DOI：
10.1007/s40778-018-0124-x
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发表时间：
2018-06-01
影响因子：
1.4
通讯作者：
Flanagan, Lisa A.
中科院分区：
文献类型：
--
作者：
Lee, Abraham P.;Aghaamoo, Mohammad;Flanagan, Lisa A.
研究目的先进技术可以以令人惊讶的方式帮助干细胞科学的发现。电动技术利用电场来询问或分离细胞，在干细胞研究中的应用已经对干细胞的功能产生了重要的见解。这些技术探测固有的细胞特性，消除了对细胞类型特异性标记的需要。最近的研究结果通过电动技术对各种干细胞类型的分析，包括造血干细胞、间充质干细胞和脂肪干细胞、神经干细胞和多能干细胞，揭示了细胞的命运特异性特征。独特的固有细胞特性足以使它们在不造成细胞损伤或毒性的情况下进行无标记浓缩。概述无标记技术在干细胞分析和分类中的成功应用为探索干细胞的基本生物学和优化其在再生医学应用中的应用开辟了新的途径。
Purpose of Review Advanced technologies can aid discoveries in stem cell science in surprising ways. The application of electrokinetic techniques, which use electric fields to interrogate or separate cells, to the study of stem cells has yielded important insights into stem cell function. These techniques probe inherent cell properties, obviating the need for cell-type specific labels.Recent Findings Analysis of a variety of stem cell types including hematopoietic, mesenchymal and adipose-derived, neural, and pluripotent stem cells by electrokinetic techniques has revealed fate-specific signatures of cells. Distinct inherent cell properties are sufficient for their label-free enrichment without causing cell damage or toxicity.Summary The successful application of label-free techniques to the analysis and sorting of stem cells open new avenues for exploring the basic biology of stem cells and optimizing their use in regenerative medicine applications.